Department of Restorative Dentistry, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
Department of Medical Pharmacology, Faculty of Medicine, Kafkas University, Kars, Turkey.
Drug Chem Toxicol. 2021 Sep;44(5):502-507. doi: 10.1080/01480545.2019.1620265. Epub 2019 May 31.
The aim of this study was to evaluate the cytotoxic and oxidative effects of the most commonly used dental restorative materials on human gingival fibroblast cells (HGFCs). HGFCs were obtained from healthy individuals. The tested restorative materials were a microhybrid resin based composite, a compomer resin, a glass ionomer cement, and an amalgam alloy. One hundred eight cylindirical samples, 10 mm in diameter and 2 mm in height, were prepared according to ISO 10993-12:2002 specifications ( = 9 in the tested subgroups). Freshly prepared and aged samples in artificial saliva at 37 °C (7 and 21 d) were placed into well plates and incubated. Wells without dental materials were constituted as the control group. After 72 h incubation period, cytotoxicity was determined using the neutral red (NR) assay. Oxidative alterations were assessed using total antioxidant capacity (TAC) and total oxidant status (TOS) assay kits. Data were analyzed using the ANOVA and LSD post hoc tests. All tested materials led to significant decreases in the cell viability rates (33-73%) compared to the control group. Glass ionomer and resin composite were found to be more cytotoxic than amalgam alloy and compomer. The highest TAC level was observed in glass ionomer after seven-day aging and these changes prevented an increase in TOS levels. Increases in TAC levels after seven-day aging in all groups exhibited significant differences with freshly prepared samples ( < 0.05). In all material groups, TOS levels of freshly prepared samples differed statistically and significantly from samples aged for 7 and 21 d ( < 0.05). The data obtained suggested that all the tested materials exhibited cytotoxic and pro-oxidant features. Freshly prepared samples caused higher TOS levels. However, oxidant status induced by materials decreased over time.
本研究旨在评估最常用的牙科修复材料对人牙龈成纤维细胞（HGFCs）的细胞毒性和氧化作用。HGFCs 从健康个体中获得。测试的修复材料是一种微混合树脂基复合材料、一种复合体树脂、一种玻璃离子水门汀和一种汞合金合金。根据 ISO 10993-12:2002 规范（在测试的亚组中为 = 9）制备了 108 个直径为 10mm、高度为 2mm 的圆柱形样本。将在 37°C 的人工唾液中新鲜制备和老化的样本（7 和 21 d）放入微孔板中孵育。没有牙科材料的孔被构造成对照组。孵育 72 h 后，使用中性红（NR）测定法测定细胞毒性。使用总抗氧化能力（TAC）和总氧化状态（TOS）测定试剂盒评估氧化变化。使用方差分析和 LSD 事后检验对数据进行分析。与对照组相比，所有测试材料均导致细胞活力率显著降低（33-73%）。玻璃离子和树脂复合材料比汞合金合金和复合体更具细胞毒性。在 7 天老化后，观察到玻璃离子的 TAC 水平最高，这些变化阻止了 TOS 水平的升高。所有组在 7 天老化后 TAC 水平的升高与新鲜制备的样品相比均表现出显著差异（ < 0.05）。在所有材料组中，新鲜制备样品的 TOS 水平与老化 7 天和 21 天的样品有统计学显著差异（ < 0.05）。获得的数据表明，所有测试材料均表现出细胞毒性和促氧化作用。新鲜制备的样品导致更高的 TOS 水平。然而，材料引起的氧化状态随时间而降低。
