Caughman W F, Caughman G B, Dominy W T, Schuster G S
Medical College of Georgia, School of Dentistry, Augusta.
J Prosthet Dent. 1990 May;63(5):513-21. doi: 10.1016/0022-3913(90)90067-m.
Because the cement interfaces of restorations can approximate the periodontium, it is critical to determine the biocompatibility of cements. In this study, the cytotoxic potential of resin luting agents on cultures of gingival fibroblasts and oral epithelial cells was evaluated for direct microscopic cytotoxicity, cell morbidity, impaired adherence, and inhibition of macromolecular synthesis. Visible effects ranged from severe toxicity with inadequately polymerized composite resin to no detectable morphologic cell damage by a glass ionomer cement, but inhibition of protein and RNA synthesis varied with the material and cell type. The glass ionomer cement demonstrated no morphologic damage, but exhibited inhibition of macromolecular synthesis in gingival fibroblasts. These results confirmed that in vitro metabolic assays are appropriate for examining the biologic effects of materials.
由于修复体的粘结剂界面可能接近牙周组织，因此确定粘结剂的生物相容性至关重要。在本研究中，评估了树脂粘结剂对牙龈成纤维细胞和口腔上皮细胞培养物的细胞毒性潜力，包括直接显微镜下的细胞毒性、细胞病变、粘附受损和大分子合成抑制。可见效应范围从聚合不完全的复合树脂的严重毒性到玻璃离子水门汀未检测到的形态学细胞损伤，但蛋白质和RNA合成的抑制因材料和细胞类型而异。玻璃离子水门汀未表现出形态学损伤，但在牙龈成纤维细胞中表现出大分子合成的抑制。这些结果证实，体外代谢试验适用于检测材料的生物学效应。
