Shaw L, Yousefi S, Dennis J W, Schauer R
Biochemisches Institut, Christian-Albrechts Universität, Kiel, Germany.
Glycoconj J. 1991 Oct;8(5):434-41. doi: 10.1007/BF00731295.
The dominant glycosylation mutants of MDAY-D2 mouse lymphoma cells, designated class 2 (D33W25 and D34W25) were selected for their resistance to the toxic effects of wheat germ agglutinin (WGA) and shown to express elevated levels of Neu5Gc. In accordance with this, the activity of CMP-Neu5Ac hydroxylase was found to be substantially higher in the mutant cells. The hydroxylase in the D33W25 mutant cells exhibited kinetic properties identical to those of the same enzyme from mouse liver. Growth rate experiments in vivo and in vitro, where the mutant cells grew more slowly at low cell densities in serum-free medium and also formed slower growing tumours in syngeneic mice, indicate that CMP-Neu5Ac hydroxylase expression may be associated with altered growth of the mutant cells.
MDAY-D2小鼠淋巴瘤细胞的主要糖基化突变体，即2类（D33W25和D34W25），因其对麦胚凝集素（WGA）毒性作用具有抗性而被筛选出来，并显示其Neu5Gc表达水平升高。据此，发现突变细胞中CMP-Neu5Ac羟化酶的活性显著更高。D33W25突变细胞中的羟化酶表现出与小鼠肝脏中同一种酶相同的动力学特性。体内和体外生长速率实验表明，突变细胞在无血清培养基中低细胞密度时生长较慢，并且在同基因小鼠中形成生长较慢的肿瘤，这表明CMP-Neu5Ac羟化酶的表达可能与突变细胞生长改变有关。
