Kerbel R S, Florian M, Man M S, Dennis J, McKenzie I F
J Natl Cancer Inst. 1980 May;64(5):1221-30.
The properties of an unusual mouse tumor capable of extremely rapid and widespread spontaneous metastatic growth were recently described; this tumor, called MDAY-D2, at first appeared to be an H-2Kk loss variant of an (A X DBA/2)F1 (H-2KkDd) sarcoma called MDAY and was obtained by serial ip passage of MDAY in DBA/2 (KdDd) mice. The studies described here were concerned with the analysis of the origin of MDAY-D2; i.e., was it a true variant or a newly induced DBA/2 tumor? Several approaches were used, most of which exploited defined cell surface alloantigenic systems as natural genetic markers. The results indicated that MDAY-D2 was indeed a newly induced DBA/2 tumor and, furthermore, that MDAY was a homozygous A-strain tumor, probably a T-cell lymphoma. Thus a) MDAY was found to be Ly-1.2+, Ly-2.2+, and Thy-1.2+, but Ly-6.2-, whereas the opposite pattern was observed with MDAY-D2; b) MDAY possessed the private and public H-2 specificities associated with H-2k and H-2Dd, but not H-2Dk [i.e., it typed as an A-strain (H-2a) tumor, not as (A X DBA/2)F1]; c) MDAY-D2 possessed private and public specificities associated with H-2Kd and H-2Dd and was found to be H-2Kk-negative [i.e., it typed as a DBA/2 (H-2d) tumor]; d) serial injection of clonally derived ouabain-resistant H-2Kk-positive MDAY cells into DBA/2 hosts led to the rapid development of an MDAY-D2 (H-2d-positive) tumor that was fully ouabain-sensitive. Several findings did not support a contaminant theory to explain induction of MDAY-D2. The rapid induction of a tumor after injection of allogeneic tumor cells may have importance in relation to oncogenesis, tumor variant formation, and tumor progression. The results showed that tumor cells themselves can be potent carcinogens.
最近描述了一种异常小鼠肿瘤的特性，该肿瘤能够进行极其快速且广泛的自发转移性生长；这种肿瘤名为MDAY-D2，最初似乎是一种名为MDAY的（A×DBA/2）F1（H-2KkDd）肉瘤的H-2Kk缺失变体，它是通过将MDAY在DBA/2（KdDd）小鼠中进行腹腔连续传代而获得的。此处所描述的研究关注于对MDAY-D2起源的分析；也就是说，它是一个真正的变体还是一个新诱导的DBA/2肿瘤？使用了几种方法，其中大多数利用已定义的细胞表面同种异体抗原系统作为天然遗传标记。结果表明MDAY-D2确实是一个新诱导的DBA/2肿瘤，此外，MDAY是一个纯合的A系肿瘤，可能是一种T细胞淋巴瘤。因此，a）发现MDAY是Ly-1.2+、Ly-2.2+和Thy-1.2+，但Ly-6.2-，而MDAY-D2则呈现相反的模式；b）MDAY具有与H-2k和H-2Dd相关的私有和共有H-2特异性，但不具有H-2Dk [即，它被鉴定为A系（H-2a）肿瘤，而不是（A×DBA/2）F1]；c）MDAY-D2具有与H-2Kd和H-2Dd相关的私有和共有特异性，并且被发现为H-2Kk阴性 [即，它被鉴定为DBA/2（H-2d）肿瘤]；d）将克隆衍生的哇巴因抗性H-2Kk阳性MDAY细胞连续注射到DBA/2宿主中导致快速发展出一个完全对哇巴因敏感的MDAY-D2（H-2d阳性）肿瘤。有几个发现不支持用污染物理论来解释MDAY-D2的诱导。注射同种异体肿瘤细胞后肿瘤的快速诱导可能在肿瘤发生、肿瘤变体形成和肿瘤进展方面具有重要意义。结果表明肿瘤细胞本身可能是强效致癌物。
