用于巨细胞病毒先天性感染产前诊断的新型商业实时荧光定量聚合酶链反应检测方法的评估
Evaluation of new commercial real-time PCR quantification assay for prenatal diagnosis of cytomegalovirus congenital infection.
作者信息
Ducroux Aurélie, Cherid Samira, Benachi Alexandra, Ville Yves, Leruez-Ville Marianne
机构信息
Laboratoire de Virologie, Hôpital Necker-Enfants-malades, 75015 Paris, France.
出版信息
J Clin Microbiol. 2008 Jun;46(6):2078-80. doi: 10.1128/JCM.02179-07. Epub 2008 Apr 16.
Abstract
A new commercial real-time human cytomegalovirus (HCMV) PCR kit was evaluated after automated DNA extraction of 153 amniotic fluids in parallel with an in-house real-time PCR assay. The commercial kit displayed 100% sensitivity/specificity compared to the "in-house" assay and was suitable for prenatal diagnosis of HCMV congenital infection.
摘要
在对153份羊水进行自动DNA提取后，使用一种新的商业实时人巨细胞病毒（HCMV）PCR试剂盒，并与内部实时PCR检测方法进行平行评估。与“内部”检测方法相比，该商业试剂盒显示出100%的灵敏度/特异性，适用于HCMV先天性感染的产前诊断。
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