Kimura Yoshiyuki, Sumiyoshi Maho, Taniguchi Masahiko, Baba Kimiye
Division of Biochemical Pharmacology, Department of Basic Medical Research, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime, 791-0295, Japan.
J Nat Med. 2008 Jul;62(3):308-13. doi: 10.1007/s11418-008-0242-3. Epub 2008 Apr 17.
In a series of studies on the search for new antitumor and antimetastatic substances from the natural medicinal plants of the Umbelliferae family, we previously reported that chalcone derivatives isolated from Angelica keisekei roots have antitumor and antimetastatic activities. In the present study, we examined the effects of a chromone glucoside cnidimoside A isolated from Cnidium japonicum whole plants on tumor growth and tumor metastasis in colon 26-bearing mice. Cnidimoside A (50 mg/kg, twice daily) significantly inhibited tumor growth and final tumor weight compared to the growth in vehicle-treated colon 26-bearing mice (control). Furthermore, the number of mice with abdominal invasion of tumors was also reduced by orally administered cnidimoside A (50 mg/kg, twice daily). In this study, the CD8(+) T Cell- and interferon (IFN)-gamma-positive cell numbers in the small intestine in the colon 26-bearing mice were significantly reduced compared with those in the normal mice, but the natural killer (NK)-positive cell number did not differ significantly between the normal and colon 26-bearing mice. The CD8(+) T-, NK and IFN-gamma-positive cell numbers in the small intestine were significantly increased by orally administered cnidimoside A (50 mg/kg, twice daily) compared to those in vehicle-treated colon 26-bearing mice. In conclusion, it seems likely that the antitumor and antimetastatic actions of cnidimoside A may be partly associated with the stimulation of immune response in the small intestine.
在一系列从伞形科天然药用植物中寻找新型抗肿瘤和抗转移物质的研究中，我们之前报道过从紫花前胡根部分离得到的查尔酮衍生物具有抗肿瘤和抗转移活性。在本研究中，我们检测了从日本芎䓖全株中分离得到的色酮糖苷蛇床子苷A对荷结肠26肿瘤小鼠肿瘤生长和转移的影响。与给予溶媒处理的荷结肠26肿瘤小鼠（对照）相比，蛇床子苷A（50mg/kg，每日两次）显著抑制了肿瘤生长和最终肿瘤重量。此外，口服蛇床子苷A（50mg/kg，每日两次）也减少了肿瘤发生腹部侵袭的小鼠数量。在本研究中，荷结肠26肿瘤小鼠小肠中的CD8(+) T细胞和干扰素（IFN）-γ阳性细胞数量与正常小鼠相比显著减少，但正常小鼠和荷结肠26肿瘤小鼠之间的自然杀伤（NK）阳性细胞数量没有显著差异。与给予溶媒处理的荷结肠26肿瘤小鼠相比，口服蛇床子苷A（50mg/kg，每日两次）使小肠中的CD8(+) T细胞、NK细胞和IFN-γ阳性细胞数量显著增加。总之，蛇床子苷A的抗肿瘤和抗转移作用可能部分与刺激小肠免疫反应有关。
