Breuer Judith, Schmid D Scott
Skin Virus Laboratory, Centre for Cutaneous Research, St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary College, London, United Kingdom.
J Infect Dis. 2008 Mar 1;197 Suppl 2:S54-7. doi: 10.1086/522140.
As with most live attenuated viral vaccines, varicella vaccine comprises a mixture of variant strains. Knowledge about the pathogenic potential of individual strains in the varicella vaccine is limited. Vaccination against chickenpox causes a usually modified varicella-like rash in a small percentage of healthy children, and vaccine virus reactivates on rare occasions to cause herpes zoster (HZ). In several published studies, our respective laboratories have analyzed genomic variation among specimens from cases of postvaccination rash and HZ in vaccine recipients, focusing on polymorphisms between vaccine Oka strains and the parental Oka strain. In most respects, these studies were in close agreement, identifying the set of wild-type markers among vaccine adverse event isolates, each occurring at similar frequencies. The same 3 universally present vaccine markers, at positions 106262, 107252, and 108111, were also identified by both laboratories. One notable difference has been the observation of mostly clonal vaccine virus among isolates examined by one laboratory and mostly mixed viruses in isolates examined by the other. In addition to reviewing and comparing our combined observations, we propose possible explanations for our contrasting findings and propose future studies to reconcile them.
与大多数减毒活病毒疫苗一样，水痘疫苗包含多种变异株的混合物。关于水痘疫苗中各个毒株的致病潜力的了解有限。接种水痘疫苗在一小部分健康儿童中通常会引起类似水痘的皮疹，并且疫苗病毒在极少数情况下会重新激活导致带状疱疹（HZ）。在几项已发表的研究中，我们各自的实验室分析了疫苗接种后皮疹和HZ病例的标本中的基因组变异，重点关注疫苗Oka株与亲本Oka株之间的多态性。在大多数方面，这些研究结果非常一致，确定了疫苗不良事件分离株中的野生型标记集，每个标记出现的频率相似。两个实验室还都鉴定出了位于106262、107252和108111位置的相同的3个普遍存在的疫苗标记。一个显著的差异是，一个实验室检测的分离株中大多是克隆疫苗病毒，而另一个实验室检测的分离株中大多是混合病毒。除了回顾和比较我们的综合观察结果外，我们还对我们相互矛盾的发现提出了可能的解释，并提出了未来的研究以协调这些发现。
