Yurdakoc A, Gunday I, Memiş D
Department of Anaesthesiology, Kirklareli Hospital, Edirne, Turkey.
Acta Anaesthesiol Scand. 2008 May;52(5):658-63. doi: 10.1111/j.1399-6576.2008.01635.x.
In a rat closed head trauma model we examined both the time course of lipid peroxidation and the effects of halothane, isoflurane, and sevoflurane on it by analysis of malondialdehyde (MDA) formation.
Animals were divided randomly into five groups: sham-operated (SO), n=18; control-closed head trauma to left frontal pole, n=18; closed head trauma model+halothane, n=18; closed head trauma model+isoflurane, n=18; and closed head trauma model+sevoflurane, n=18. Halothane, isoflurane, or sevoflurane were applied 15 min after trauma for 30 min. Rats were euthanized 1,3, and 5 h after the inhalation agents. Brain tissue samples were taken 5 mm from the left and right frontal poles. MDA was considered to reflect the degree of lipid peroxidation.
MDA concentrations were greater in the control, halothane, sevoflurane, and isoflurane groups than in SO animals (P<0.001). No statistical difference between the hemispheres was found between the halothane, isoflurane, or sevoflurane groups, but MDA levels were lower with isoflurane than in the halothane, sevoflurane, and control groups at 1, 3, and 5 h (P<0.001). MDA levels were higher as compared with the halothane and sevoflurane groups at 1 h but not at 3 or 5 h (P<0.001).
MDA levels with the isoflurane group were lower than in the other trauma groups, which suggest that isoflurane, given after closed head trauma, might be protective against lipid peroxidation of cerebral injury.
在大鼠闭合性颅脑创伤模型中，我们通过分析丙二醛（MDA）的形成来研究脂质过氧化的时间进程以及氟烷、异氟烷和七氟烷对其的影响。
将动物随机分为五组：假手术组（SO），n = 18；左额极闭合性颅脑创伤对照组，n = 18；闭合性颅脑创伤模型+氟烷组，n = 18；闭合性颅脑创伤模型+异氟烷组，n = 18；闭合性颅脑创伤模型+七氟烷组，n = 18。创伤后15分钟给予氟烷、异氟烷或七氟烷，持续30分钟。吸入麻醉剂后1、3和5小时对大鼠实施安乐死。从左右额极5毫米处采集脑组织样本。MDA被认为可反映脂质过氧化程度。
对照组、氟烷组、七氟烷组和异氟烷组的MDA浓度高于假手术组动物（P < 0.001）。氟烷组、异氟烷组或七氟烷组的半球间未发现统计学差异，但在1、3和5小时时，异氟烷组的MDA水平低于氟烷组、七氟烷组和对照组（P < 0.
