Qin Zhaojun, Lv En, Zhan Leyun, Xing Xiangfei, Jiang Jianli, Zhang Min
Department of Anesthesiology, Three Gorges University People's Hospital, Yichang, China, No. 4, Hudi Street, Xiling District, Yichang 443000, Hubei, People's Republic of China.
BMC Anesthesiol. 2014 Apr 16;14:28. doi: 10.1186/1471-2253-14-28. eCollection 2014.
Emulsified isoflurane (EIso) is a novel intravenous general anesthetic, which can provide rapid anesthetic induction and recovery. EIso preconditioning could attenuate heart, lung and liver ischemia/reperfusion (I/R) injury. We tested the hypothesis that intravenous pretreatment with EIso would protect kidneys against I/R injury by inhibiting systemic inflammatory responses and improving renal antioxidative ability.
RATS WERE RANDOMLY DIVIDED INTO THESE SIX GROUPS: sham, I/R, intralipid, 1, 2 or 4 ml/kg EIso. Rats were subjected to 45 min left renal pedicle occlusion followed by 3 h reperfusion after right nephrectomy. Rat were treated with intravenous 8% EIso with 1, 2 or 4 ml/kg, or 30% intralipid with 2 ml/kg for 30 min before ischemia, respectively. After reperfusion, renal functional parameters, serum mediator concentrations and markers of oxidative stress in kidney tissues were determined, and renal histopathological analysis were performed.
Serum creatinine, blood urea nitrogen, cystatin c, tumor necrosis factor-α, interleukin-6, and interleukin-10 concentrations were significantly increased after renal I/R as compared to the sham group. So was renal tissue MDA content and histological scores, but renal tissue SOD activity was decreased. Additionally, severe morphological damages were observed in these study groups. In contrast, 2 or 4 ml/kg EIso reduced serum creatinine, blood urea nitrogen, cystatin c, tumor necrosis factor-α, and interleukin-6 levels, decreased renal tissue MDA content and histological scores, increased serum interleukin-10 level and tissue SOD activity as compared to the I/R, intralipid and 1 ml/kg EIso groups. Renal morphological damages were alleviated after pretreatment of 2 or 4 ml/kg EIso.
Intravenous EIso produces preconditioning against renal I/R injury in rats, which might be mediated by attenuating inflammation and increasing antioxidation ability.
乳化异氟烷（EIso）是一种新型静脉全身麻醉剂，可实现快速麻醉诱导和苏醒。EIso预处理可减轻心脏、肺和肝脏的缺血/再灌注（I/R）损伤。我们验证了以下假设：EIso静脉预处理可通过抑制全身炎症反应和提高肾脏抗氧化能力来保护肾脏免受I/R损伤。
将大鼠随机分为六组：假手术组、I/R组、脂质乳剂组、1、2或4 ml/kg EIso组。大鼠在右肾切除术后进行45分钟的左肾蒂阻断，然后再灌注3小时。分别在缺血前30分钟用1、2或4 ml/kg的8% EIso或2 ml/kg的30%脂质乳剂对大鼠进行静脉注射治疗。再灌注后，测定肾功能参数、血清介质浓度和肾组织氧化应激标志物，并进行肾脏组织病理学分析。
与假手术组相比，肾脏I/R后血清肌酐、血尿素氮、胱抑素c、肿瘤坏死因子-α、白细胞介素-6和白细胞介素-10浓度显著升高。肾组织丙二醛含量和组织学评分也升高，但肾组织超氧化物歧化酶活性降低。此外，在这些研究组中观察到严重的形态学损伤。相比之下，与I/R组、脂质乳剂组和1 ml/kg EIso组相比，2或4 ml/kg EIso可降低血清肌酐、血尿素氮、胱抑素c、肿瘤坏死因子-α和白细胞介素-6水平，降低肾组织丙二醛含量和组织学评分，提高血清白细胞介素-10水平和组织超氧化物歧化酶活性。2或4 ml/kg EIso预处理后肾形态学损伤减轻。
静脉注射EIso可对大鼠肾脏I/R损伤产生预处理作用，这可能是通过减轻炎症和提高抗氧化能力来介导的。
