Ozturk Erdogan, Demirbilek Semra, Kadir But A, Saricicek Vahap, Gulec Mukaddes, Akyol Omer, Ozcan Ersoy M
Inonu University, Faculty of Medicine, Department of Anesthesia, Turgut Ozal Medical Center, 44315 Malatya, Turkey.
Prog Neuropsychopharmacol Biol Psychiatry. 2005 Jul;29(6):922-7. doi: 10.1016/j.pnpbp.2005.04.028.
Reactive oxygen species play a role during brain injury due to closed head trauma. Enzymatic or nonenzymatic antioxidants may protect brain tissue against oxidative damage. The present study was performed to assess the changes of endogenous indices of oxidative stress in serum from rats subjected to head trauma and whether treatment with propofol and/or erythropoietin (EPO) modifies the levels of endogenous indices of oxidative stress. For these purposes, female Wistar Albino rats were divided into five groups: non-traumatic sham group, trauma performed control, trauma with propofol (i.p.), trauma with EPO (i.p.) and trauma with propofol and EPO performed study groups. At the end of the experimental procedure, blood was taken by cardiac puncture to determine superoxide dismutase (SOD) and xanthine oxidase (XO) activities as well as malondialdehyde (MDA) and nitric oxide (NO) levels in serum. Serum MDA level of control traumatic brain injury (TBI) group was significantly higher than sham operation group (p<0.012). Serum MDA levels in propofol, EPO and propofol+EPO groups were found to be decreased in comparison with control group (p<0.039, p<0.030 and p<0.018, respectively). Serum NO level was found to be increased in TBI group, but difference was not statistically significant when compared to sham-operated group (p=0.092). Propofol, EPO and propofol+EPO administration efficiently reduced serum NO levels to reach sham-operated group (p<0.002, p<0.001 and p<0.015, respectively). These results suggested that acute administration of both propofol and EPO altered the indices of oxidative stress similarly against brain injury due to trauma.
活性氧在闭合性颅脑创伤所致脑损伤过程中发挥作用。酶促或非酶促抗氧化剂可能保护脑组织免受氧化损伤。本研究旨在评估颅脑创伤大鼠血清中氧化应激内源性指标的变化，以及丙泊酚和/或促红细胞生成素（EPO）治疗是否会改变氧化应激内源性指标的水平。为此，将雌性Wistar白化大鼠分为五组：非创伤性假手术组、创伤对照组、丙泊酚腹腔注射创伤组、EPO腹腔注射创伤组和丙泊酚与EPO联合注射创伤研究组。实验结束时，通过心脏穿刺采血以测定血清中超氧化物歧化酶（SOD）和黄嘌呤氧化酶（XO）活性以及丙二醛（MDA）和一氧化氮（NO）水平。创伤性脑损伤（TBI）对照组血清MDA水平显著高于假手术组（p<0.012）。与对照组相比，丙泊酚组、EPO组和丙泊酚+EPO组血清MDA水平均降低（分别为p<0.039、p<0.030和p<0.018）。TBI组血清NO水平升高，但与假手术组相比差异无统计学意义（p=0.092）。丙泊酚、EPO和丙泊酚+EPO给药均有效降低血清NO水平，使其达到假手术组水平（分别为p<0.002、p<0.001和p<
