具有氧化还原活性的新型最小核酶：从圆酵母RNA中分离出的5-羟基尿苷、8-羟基鸟苷和8-羟基腺苷。
Novel minimum ribozymes with oxidoreduction activity: 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine isolated from Torula yeast RNA.
作者信息
Yanagawa H, Ogawa Y, Ueno M
机构信息
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
出版信息
Nucleic Acids Symp Ser. 1991(25):113-4.
Three nucleosides catalyzing the oxidoreduction of NADH and K3Fe(CN)6 were isolated from Torula yeast RNA and also obtained by a series of steps: SDS-phenol extraction, nuclease P1 digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS column. Their chemical structures were clearly determined as 5-hydroxyuridine, 8-hydroxyguanosine, and 8-hydroxyadenosine from the results of FAB-MS, 1H and 13C-NMR spectroscopies.
从产朊假丝酵母RNA中分离出了三种催化NADH和铁氰化钾氧化还原反应的核苷，并且通过一系列步骤获得了这些核苷：十二烷基硫酸钠-苯酚萃取、核酸酶P1消化、碱性磷酸酶消化、阴离子交换色谱法以及在十八烷基硅烷键合硅胶柱上进行的高效液相色谱法。根据快原子轰击质谱、氢核磁共振和碳-13核磁共振光谱的结果，明确确定了它们的化学结构分别为5-羟基尿苷、8-羟基鸟苷和8-羟基腺苷。
Zotero 插件