寻找新型RNA催化剂。一种具有氧化还原酶活性的RNA组分。
Search for novel RNA catalysts. An RNA component with oxidoreductase activity.
作者信息
Yanagawa H, Ogawa Y, Ueno M, Sasaki K, Sato T
机构信息
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
出版信息
Nucleic Acids Symp Ser. 1990(22):61-2.
PMID:2101915
Abstract
A novel RNA component with oxidoreductase activity (diaphorase activity) has been purified from an RNA fraction of Torula yeast. The RNA component was obtained in a 0.05% yield by a series of steps, SDS-phenol extraction, nuclease P1 digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS-column.
摘要
一种具有氧化还原酶活性（黄递酶活性）的新型RNA组分已从球拟酵母的RNA组分中纯化出来。通过一系列步骤，即SDS-苯酚提取、核酸酶P1消化、碱性磷酸酶消化、阴离子交换色谱法以及在ODS柱上进行高效液相色谱法，以0.05%的产率获得了该RNA组分。
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