Abrahamse P A, Dijkstra J, Vlaeminck B, Tamminga S
Animal Nutrition Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH Wageningen, the Netherlands.
J Dairy Sci. 2008 May;91(5):2033-45. doi: 10.3168/jds.2007-0579.
Twenty Holstein cows were blocked in 2 groups according to milk yield to evaluate the effect of frequency of allocation to new grazing plots on pasture intake, grazing behavior, rumen characteristics, and milk yield. The 2 treatments, daily allocation to 0.125-ha plots (1D) or allocation every 4 d to 0.5-ha plots (4D) of Lolium perenne L., were tested in a randomized block design (2 rotations with 3 or 4 measuring periods of 4 d each) with mixed model analysis accounting for repeated measures. There were no differences in the chemical composition of offered pasture and in pasture dry matter intake (DMI) between 1D and 4D. However, an interaction between treatment and rotation indicated a difference in pasture DMI between treatments during the first rotation (4D, 16.5 vs. 1D, 18.3 kg/d) but not during the second rotation (4D, 15.0 vs. 1D, 14.7 kg/d), possibly a result of a greater pasture mass in the first rotation. Grazing time (average 562 min/d) and ruminating time (average 468 min/d), observed using IGER graze recorders, were similar between treatments, but grazing time increased numerically (549 to 568 min/d), and ruminating time decreased linearly (471 to 450 min/d) within periods in the 4D treatment. Mean rumen pH (6.16 vs. 6.05) and rumen NH(3)-N concentration (113.7 vs. 90.1 mg/L) were higher in 4D than in 1D, and total volatile fatty acid (VFA) concentrations did not differ. Molar proportions of VFA, except butyrate, differed between treatments, causing the nonglucogenic to glucogenic VFA ratio to be greater in 4D than in 1D. Within days in the 4D treatment, the molar proportion of acetate increased and those of all other VFA decreased linearly. Rumen NH(3)-N concentration within the 4D treatment declined quadratically from 170.3 mg/L on d 1 to 80.7 mg/L on d 4. In contrast to rumen NH(3)-N concentration, milk urea content did not differ between treatments, but decreased quadratically from d 1 to 4 in the 4D treatment (from 26.7 to 20.7 mg/dL). Mean fat- and protein-corrected milk was greater in 1D than in 4D (23.5 vs. 22.8 kg/d), mainly due to a difference in milk yield (24.5 vs. 23.7 kg/d). Fat and protein content were slightly lower in the 1D than in the 4D treatment (3.66 vs. 3.76% and 3.28 vs. 3.34%, respectively). This study confirmed that increasing pasture allocation frequency from once every 4 d to every day improved milk production in grazing dairy cows, especially when offered pasture was high.
根据产奶量将20头荷斯坦奶牛分为2组,以评估新放牧地分配频率对牧场采食量、放牧行为、瘤胃特征和产奶量的影响。两种处理方式,即每日分配到0.125公顷的地块(1D)或每4天分配到0.5公顷的多年生黑麦草地块(4D),采用随机区组设计(2次轮作,每次有3或4个为期4天的测量期)进行测试,并使用混合模型分析考虑重复测量。1D和4D之间提供的牧场化学成分和牧场干物质采食量(DMI)没有差异。然而,处理与轮作之间的相互作用表明,在第一次轮作期间,处理之间的牧场DMI存在差异(4D,16.5对1D,18.3千克/天),但在第二次轮作期间没有差异(4D,15.0对1D,14.7千克/天),这可能是由于第一次轮作中牧场质量更高的结果。使用IGER放牧记录器观察到,处理之间的放牧时间(平均562分钟/天)和反刍时间(平均468分钟/天)相似,但在4D处理中,放牧时间在各时间段内数值上有所增加(549至568分钟/天),反刍时间呈线性下降(471至450分钟/天)。4D的平均瘤胃pH值(6.16对6.05)和瘤胃NH₃-N浓度(113.7对90.1毫克/升)高于1D,总挥发性脂肪酸(VFA)浓度没有差异。除丁酸盐外,VFA的摩尔比例在处理之间有所不同,导致4D中不可生糖VFA与可生糖VFA的比例高于1D。在4D处理中的各天内,乙酸盐的摩尔比例增加,所有其他VFA的摩尔比例呈线性下降。4D处理中的瘤胃NH₃-N浓度从第1天的170.3毫克/升呈二次方下降至第4天的80.7毫克/升。与瘤胃NH₃-N浓度相反,处理之间的牛奶尿素含量没有差异,但在4D处理中从第1天到第4天呈二次方下降(从26.7降至20.7毫克/分升)。1D的平均脂肪和蛋白质校正乳产量高于4D(23.5对22.8千克/天),主要是由于产奶量的差异(24.5对23.7千克/天)。1D的脂肪和蛋白质含量略低于4D处理(分别为3.66%对3.76%和3.28%对3.34%)。本研究证实,将牧场分配频率从每4天一次增加到每天一次可提高放牧奶牛的产奶量,尤其是在提供的牧场质量较高时。