[核因子-κB和细胞间黏附分子-1 mRNA在变应性鼻炎鼻黏膜中的表达及意义]

[Expression and significance of nuclear factor-kappaB and ICAM-1 mRNA in nasal mucosa of allergic rhinitis].

作者信息

Zhao Jiandong, Wang Yanjun, Kong Weijia

机构信息

Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Jan;22(2):57-60.

PMID:18422166

Abstract

OBJECTIVE

To clarify the pathogenesis of allergic rhinitis (AR) by detecting the activation of NF-kappaB and the expression of ICAM-1 mRNA in nasal mucosa of the patients with AR.

METHOD

AR patients were collected as experimental group and deflection of nasal septum patients as control group. Immunohistochemistry methods were used to examine the expression of NF-kappaB p50 and p65 subunits in the middle turbinate mucous tunic. ICAM-1 mRNA were detected by means of RT-PCR.

RESULT

NF-kappaB was strongly expressed in AR group. NF-kappaB p50 existed in cytoplasm and nucleus. NF-kappaB p65 was mainly expressed in cytoplasm and less in nucleus. While NF-kappaB was expressed little in control group. The difference of NF-kappaB p50 and NF-kappaB p65 expression between two groups were significantly (P < 0.01). ICAM-1 mRNA was more strongly expressed in AR group (P < 0.01) while feebly expressed in deflection of nasal septum group. The expression of ICAM-1 mRNA were significantly correlated with that of NF-kappaB p50 and NF-kappaB p65 (r = 0.899 5, P < 0.01; r = 0.7601, P < 0.01).

CONCLUSION

NF-kappaB plays a key role in AR. The excessively activated NF-kappaB promotes the transcription of ICAM-1 mRNA. ICAM-1 relates to the pathogenesis and development of AR.

摘要

目的

通过检测变应性鼻炎（AR）患者鼻黏膜中核因子κB（NF-κB）的激活情况及细胞间黏附分子-1（ICAM-1）mRNA的表达，以阐明AR的发病机制。

方法

收集AR患者作为实验组，鼻中隔偏曲患者作为对照组。采用免疫组织化学方法检测中鼻甲黏膜中NF-κB p50和p65亚基的表达。通过逆转录聚合酶链反应（RT-PCR）检测ICAM-1 mRNA。

结果

NF-κB在AR组中高表达。NF-κB p50存在于细胞质和细胞核中。NF-κB p65主要表达于细胞质，细胞核中较少。而NF-κB在对照组中表达较少。两组间NF-κB p50和NF-κB p65表达差异有统计学意义（P<0.01）。ICAM-1 mRNA在AR组中表达更强（P<0.01），而在鼻中隔偏曲组中表达较弱。ICAM-1 mRNA的表达与NF-κB p50和NF-κB p65的表达显著相关（r=0.899 5，P<0.01；r=0.7601，P<0.01）。