核因子-κB p50 和 p65 的表达及其在变应性鼻炎患者鼻黏膜细胞间黏附分子-1 mRNA 上调中的意义。
Expressions of nuclear factor-kappa B p50 and p65 and their significance in the up-regulation of intercellular cell adhesion molecule-1 mRNA in the nasal mucosa of allergic rhinitis patients.
机构信息
Department of Otolaryngology, Head and Neck Surgery, The Fourth People's Hospital of Ji'nan, 50# Teacher Training Road, Ji'nan 250031, Shandong, China.
出版信息
Eur Arch Otorhinolaryngol. 2013 Mar;270(4):1329-34. doi: 10.1007/s00405-012-2136-y. Epub 2012 Oct 2.
The purpose of the study was to investigate expressions of nuclear factor-kappa B (NF-κB) and intercellular cell adhesion molecule-1 mRNA (ICAM-1 mRNA) in the nasal mucosa of allergic rhinitis (AR) patients. Expressions of NF-κB and ICAM-1 mRNA were studied using immunohistochemistry and reverse transcription-PCR (RT-PCR) in AR tissues and corresponding normal nasal mucosa. The correlation between NF-κB and ICAM-1 mRNA was studied using linear correlation analysis. The results of immunohistochemistry showed that expression of NF-κB was significantly up-regulated in the nasal mucosa of AR compared with that in normal tissue (P < 0.01), over-expression of NF-κB p50 was found in the cytoplasm and nucleus (P < 0.01), and NF-κB p65 was mainly expressed in the cytoplasm (P < 0.01). ICAM-1 mRNA was strongly expressed in the nasal mucosa of AR compared with that in normal tissue as shown by RT-PCR (P < 0.01). Up-regulation of ICAM-1 mRNA was significantly correlated with over-expressions of NF-κB p50 and NF-κB p65 (r = 0.8995, P < 0.01; r = 0.7601, P < 0.01). In conclusion, NF-κB plays a key role in AR. Excessively activated NF-κB promotes the transcription of ICAM-1 mRNA. ICAM-1 is related to the pathogenesis and development of AR.
本研究旨在探讨核因子-κB（NF-κB）和细胞间黏附分子-1mRNA（ICAM-1mRNA）在变应性鼻炎（AR）患者鼻黏膜中的表达。采用免疫组织化学和逆转录-聚合酶链反应（RT-PCR）方法研究 AR 组织和相应正常鼻黏膜中 NF-κB 和 ICAM-1mRNA 的表达。采用线性相关分析研究 NF-κB 与 ICAM-1mRNA 的相关性。免疫组织化学结果显示，与正常组织相比，AR 患者鼻黏膜中 NF-κB 的表达明显上调（P < 0.01），NF-κB p50 在细胞质和细胞核中过度表达（P < 0.01），NF-κB p65 主要在细胞质中表达（P < 0.01）。RT-PCR 显示 AR 患者鼻黏膜中 ICAM-1mRNA 强烈表达，与正常组织相比差异有统计学意义（P < 0.01）。ICAM-1mRNA 的上调与 NF-κB p50 和 NF-κB p65 的过度表达呈显著相关（r = 0.8995，P < 0.01；r = 0.7601，P < 0.01）。综上所述，NF-κB 在 AR 中起关键作用。过度激活的 NF-κB 促进 ICAM-1mRNA 的转录。ICAM-1 与 AR 的发病机制和发展有关。
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