Carlile Thomas M, Amon Angelika
Center for Cancer Research, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
Cell. 2008 Apr 18;133(2):280-91. doi: 10.1016/j.cell.2008.02.032.
In budding yeast, key meiotic events such as DNA replication, recombination, and the meiotic divisions are controlled by Clb cyclin-dependent kinases (Clb-CDKs). Using a novel synchronization procedure, we have characterized the activity of these Clb-CDKs and observed a surprising diversity in their regulation during the meiotic divisions. Clb1-CDK activity is restricted to meiosis I, and Clb3-CDK activity to meiosis II, through 5'UTR-mediated translational control of its transcript. The analysis of cells inappropriately producing Clb3-CDKs during meiosis I furthermore defines Clb3 as an inhibitor of the meiosis I chromosome segregation program. Our results demonstrate an essential role for Clb-CDK regulation in establishing the meiotic chromosome segregation pattern.
在出芽酵母中,关键的减数分裂事件,如DNA复制、重组和减数分裂,由Clb细胞周期蛋白依赖性激酶(Clb-CDKs)控制。通过一种新颖的同步程序,我们对这些Clb-CDKs的活性进行了表征,并观察到它们在减数分裂过程中的调控存在惊人的多样性。通过其转录本的5'UTR介导的翻译控制,Clb1-CDK活性仅限于减数分裂I,而Clb3-CDK活性仅限于减数分裂II。对在减数分裂I期间不适当产生Clb3-CDKs的细胞进行的分析进一步将Clb3定义为减数分裂I染色体分离程序的抑制剂。我们的结果证明了Clb-CDK调控在建立减数分裂染色体分离模式中的重要作用。