Wutzen J
Cell Pathophysiology Department, Medical Centre of Postgraduate Education, Warszawa, Poland.
Mater Med Pol. 1991 Apr-Jun;23(2):92-6.
Methotrexate administration intraperitoneally in doses of 1 mg/kg body weight twice weekly for six weeks produced in the myocardial fibres of these animals slight focal lesions from the third week of the experiment on with the appearance of isolated giant mitochondria of bizarre shapes with electron-dense membranes containing granules, or groups of mitochondria with swollen matrix and disintegration or, partial breakdown of mitochondrial cristae, numerous lysosomes and less numerous autophagosomes, considerable lysis of myofibrils and myofilaments, widening of the tubules of the sarcoplasmic reticulum and T-tubules, presence of few fat droplets, widening of intercalated discs, rarely cytoplasm swelling. From the fifth week on foci of cardiomyocyte fibrosis, in some places swelling of vascular endothelial cells, and a very considerable rise in the number of collagen cells in extracellular spaces. Simultaneous low-magnesium diet and methotrexate caused earlier, from the second week on, and greater intensity of focal necrotic and degenerative changes of cardiomyocytes.
以1毫克/千克体重的剂量每周两次腹腔注射甲氨蝶呤，持续六周，从实验第三周起，这些动物的心肌纤维出现轻微局灶性病变，表现为出现形状怪异的孤立巨大线粒体，其膜电子密度高且含有颗粒，或线粒体群基质肿胀、线粒体嵴解体或部分断裂，有大量溶酶体和数量较少的自噬体，肌原纤维和肌丝大量溶解，肌浆网和T小管的小管增宽，有少量脂肪滴，闰盘增宽，很少见细胞质肿胀。从第五周起出现心肌细胞纤维化灶，有些地方血管内皮细胞肿胀，细胞外间隙胶原细胞数量大幅增加。同时采用低镁饮食和甲氨蝶呤会导致从第二周起更早出现且心肌细胞局灶性坏死和退行性变化的强度更大。
