Wutzen J
Department of Cell Pathophysiology, Medical Centre of Postgraduate Education, Warszawa, Poland.
Mater Med Pol. 1991 Oct-Dec;23(4):290-5.
Cyclophosphamide administration in 20 mg/kg doses intraperitoneally at intervals of 6 days for 6 weeks produced the appearance of slight focal changes in myocardial fibres from the third week on, with dilatation of T-tubules and smooth endoplasmic reticulum channels with electron-dense deposits in them, with the appearance of numerous fat droplets in myocardial fibres, slight mitochondrial changes, with swelling and the appearance of giant or bizarre shaped mitochondria with electron-dense granules and lamellae in them, with numerous lysosomes and some increase in the number of autophagosomes, focal widening of intercalated discs, lysis of myofilaments and myofibrils, cytoplasm swelling, focal necrosis of some myocardial fibres in the 6th week of the experiment, marked swelling of vascular endothelial cells, and a very great rise in the number of collagen fibres. Simultaneous administration of low-magnesium diet and cyclophosphamide caused a greater intensity and an earlier appearance from the 2nd week on, of the disseminated degenerative and necrotic changes in myocardial fibres.
以20mg/kg的剂量腹腔注射环磷酰胺，每隔6天注射一次，持续6周，从第三周开始，心肌纤维出现轻微的局灶性变化，包括T小管和光滑内质网通道扩张，其中有电子致密沉积物，心肌纤维中出现大量脂肪滴，线粒体有轻微变化，表现为肿胀以及出现含有电子致密颗粒和板层的巨大或奇形怪状的线粒体，有大量溶酶体，自噬体数量略有增加，闰盘局灶性增宽，肌丝和肌原纤维溶解，细胞质肿胀，在实验第6周一些心肌纤维出现局灶性坏死，血管内皮细胞明显肿胀，胶原纤维数量大幅增加。同时给予低镁饮食和环磷酰胺会使心肌纤维弥漫性变性和坏死变化的强度更大，且从第2周开始更早出现。
