Patterson D J, Sleigh M A
J Protozool. 1976 Aug;23(3):410-7. doi: 10.1111/j.1550-7408.1976.tb03796.x.
The behavior of the contractile vacuole of Tetrahymena pyriformis W has been recorded and analyzed quantitatively by cinephotography. The vacuole fills in a stepwise fashion by the confluence of ampullae which appear regularly at the beginning of systole and whose membranes are continuous with that of the contractile vacuole throughout the cycle. The vacuole may subsequently fill slowly by a means not discernible by light microscopy. The vacuole rounds up at the beginning of systole and shortly thereafter the ampullae reappear around the perimeter of the vacuole. They are expanded by fluid forced into them from the vacuole. Round-up and the mode of growth of the ampullae indicate that the contractile vacuole is truly contractile. Expulsion occurs soon after the appearance of the ampullae and terminates the cycle. Contraction is initiated at regular intervals by a timing mechanism which is independent of the size of the vacuole. Suitable terminology to describe the structure and behavior of the contractile vacuole is discussed.
利用电影摄影技术对梨形四膜虫W收缩泡的行为进行了记录和定量分析。收缩泡通过壶腹的融合以逐步的方式充盈，壶腹在收缩期开始时规律出现，其膜在整个周期中与收缩泡的膜连续。随后，收缩泡可能通过光学显微镜无法辨别的方式缓慢充盈。收缩期开始时，收缩泡变圆，此后不久，壶腹再次出现在收缩泡周围。它们因从收缩泡中挤入的液体而膨胀。收缩泡变圆以及壶腹的生长方式表明收缩泡确实具有收缩性。壶腹出现后不久即发生排出，从而结束该周期。收缩由一个定时机制以规则的间隔启动，该机制与收缩泡的大小无关。文中讨论了用于描述收缩泡结构和行为的合适术语。
