Ng S F, Frankel J
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1115-9. doi: 10.1073/pnas.74.3.1115.
With quasi-surgical techniques, longitudinal somatic ciliary rows in Tetrahymena pyriformis have been rotated 180 degrees. New structures formed in the rotated ciliary rows during growth and reproduction are disposed 180 degrees opposite to their normal positions or orientations, confirming the earlier findings of Beisson and Sonneborn on Paramecium. However, during cell fission the rotated ciliary rows exhibit abnormality in orientation along the fission zone; the configuration of these rows near the anterior end of the posterior product of fission is consequently affected. Rotated ciliary rows have been employed as a tool in the analysis of morphogenetic problems: (a) The contractile vacuole pore is normally located on the left side of a ciliary row; but it is on the right of inverted rows. Hence, the morphogenetic properties of the two sides of the ciliary row associated with the contractile vacuole pore are different and this difference is the sole determinative factor as to the side of the ciliary row on which the contractile vacuole pore is located. (b) The process that generates the rotated ciliary rows frequently also brings about the implantation of an extra band of longitudinal microtubules at a specific site on the cell surface. This extra structure is inheritable, which opens up opportunities for the study of microtubular assembly in vivo.
利用准手术技术，梨形四膜虫的纵向体纤毛列被旋转了180度。在生长和繁殖过程中，旋转纤毛列中形成的新结构相对于其正常位置或方向呈180度反向排列，这证实了贝松和索恩伯恩早期关于草履虫的研究结果。然而，在细胞分裂期间，旋转的纤毛列在沿分裂带的方向上表现出异常；因此，这些纤毛列在分裂后产物前端附近的形态受到影响。旋转纤毛列已被用作分析形态发生问题的工具：（a）收缩泡孔通常位于纤毛列的左侧；但在倒置的纤毛列上则位于右侧。因此，与收缩泡孔相关的纤毛列两侧的形态发生特性不同，这种差异是决定收缩泡孔位于纤毛列哪一侧的唯一决定性因素。（b）产生旋转纤毛列的过程通常还会在细胞表面的特定位置植入一条额外的纵向微管带。这种额外的结构是可遗传的，这为体内微管组装的研究提供了机会。
