Curr Protoc Hum Genet. 2001 May;Appendix 3:Appendix 3C. doi: 10.1002/0471142905.hga03cs00.
This appendix presents protocols for phenol extraction and ethanol precipitation, methods that are routinely used to isolate DNA from biological sources or enzymatic reaction mixtures, to concentrate DNA samples, and to change from one solvent system to another. DNA isolation from simple aqueous solutions may be accomplished by ethanol precipitation alone.
本附录介绍了酚抽提和乙醇沉淀的实验方案,这些方法常用于从生物来源或酶促反应混合物中分离DNA、浓缩DNA样品以及从一种溶剂系统转换到另一种溶剂系统。从简单水溶液中分离DNA仅通过乙醇沉淀即可完成。