从水溶液中纯化和浓缩DNA。
Purification and concentration of DNA from aqueous solutions.
作者信息
Moore D
机构信息
Baylor College of Medicine, Houston, Texas, USA.
出版信息
Curr Protoc Protein Sci. 2001 May;Appendix 4:Appendix 4E. doi: 10.1002/0471140864.psa04es13.
PMID:18429080
Abstract
This unit presents basic procedures for manipulating solutions of single- or double-stranded DNA through purification and concentration steps. Phenol extraction with ethanol precipitation is described along with an alternate procedure of precipitating DNA with isopropanol. A Support Protocol describes concentration of DNA using butanol.
摘要
本单元介绍了通过纯化和浓缩步骤处理单链或双链DNA溶液的基本程序。描述了用乙醇沉淀进行酚抽提以及用异丙醇沉淀DNA的替代程序。一个支持方案描述了使用丁醇浓缩DNA的方法。
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