Albright L M, Slatko B E
New England Biolabs, Beverly, Massachusetts, USA.
Curr Protoc Nucleic Acid Chem. 2001 May;Appendix 3:Appendix 3B. doi: 10.1002/0471142700.nca03bs00.
Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single-stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all footprinting protocols. Thicker gels are often used to purify oligonucleotides. This appendix describes the pouring, running, and processing of a typical sequencing gel, which is 40 cm long with a uniform thickness of 0.4 mm, containing 7 M urea and 4% to 8% acrylamide.
含有高浓度尿素作为变性剂的薄聚丙烯酰胺凝胶，能够分离长度相差仅一个核苷酸的短（<500个核苷酸）DNA或RNA单链片段。这种凝胶特别适合核酸序列分析，例如所有足迹分析方案都需要这种分析。较厚的凝胶通常用于纯化寡核苷酸。本附录描述了一种典型测序凝胶的灌制、运行和处理方法，该凝胶长40厘米，厚度均匀为0.4毫米，含有7M尿素和4%至8%的丙烯酰胺。
