通过RNA指纹图谱探究RNA折叠途径
Probing RNA folding pathways by RNA fingerprinting.
作者信息
Woodson S A
机构信息
Johns Hopkins University, Baltimore, Maryland, USA.
出版信息
Curr Protoc Nucleic Acid Chem. 2001 May;Chapter 11:Unit 11.4. doi: 10.1002/0471142700.nc1104s02.
Abstract
This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution-based methods. Conformers that have been separated and immobilized in the gel matrix can be used to studying catalytic activity with or without being eluted from the gel. The method can be applied to a wide varied of catalytic RNAs and RNA-protein complexes.
摘要
本单元提供了使用天然聚丙烯酰胺凝胶电泳来区分RNA折叠和去折叠构象体的实验方案。它对于研究在几分钟或几秒钟内能够相互转换的构象体很有用，而这些构象体用基于溶液的方法很难研究。在凝胶基质中分离并固定的构象体可用于研究催化活性，无论其是否从凝胶中洗脱出来。该方法可应用于多种催化RNA和RNA-蛋白质复合物。
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