Eperon L P, Graham I R, Griffiths A D, Eperon I C
Department of Biochemistry, University of Leicester, England.
Cell. 1988 Jul 29;54(3):393-401. doi: 10.1016/0092-8674(88)90202-4.
The use in vivo of an alternative 5' splice site sequestered within a short stem of potential RNA secondary structure is determined by the length of the loop. Above a threshold length of loop, the alternative site is used despite the potential structure. In contrast, the alternative site is used very little or not at all during splicing in vitro with all lengths of loop that we have tested. A model is proposed which suggests that pre-mRNA is free to fold only within a limited period after transcription.
在体内，潜在RNA二级结构短茎内隔离的替代5'剪接位点的使用取决于环的长度。超过环的阈值长度时，尽管存在潜在结构，替代位点仍会被使用。相比之下，在我们测试的所有环长度的体外剪接过程中，替代位点很少被使用或根本不被使用。提出了一个模型，该模型表明前体mRNA在转录后的有限时间内才可以自由折叠。
