使用化学试剂和酶探测RNA结构。
Probing RNA structure with chemical reagents and enzymes.
作者信息
Ziehler W A, Engelke D R
机构信息
University of Michigan, Ann Arbor, Michigan, USA.
出版信息
Curr Protoc Nucleic Acid Chem. 2001 May;Chapter 6:Unit 6.1. doi: 10.1002/0471142700.nc0601s00.
Abstract
This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension detection protocols are included.
摘要
本单元全面介绍了可用于检测RNA二级和三级结构的最有用的化学和酶探针。介绍了使用硫酸二甲酯、焦碳酸二乙酯、乙基亚硝基脲、乙二醛、CMCT和核酸酶的足迹法。对于化学探针，还包括链断裂和引物延伸检测方案。
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