Romesberg Floyd E, Yu Chengzhi, Matsuda Shigeo, Henry Allison A
The Scripps Research Institute, La Jolla, California, USA.
Curr Protoc Nucleic Acid Chem. 2002 Nov;Chapter 1:Unit 1.5. doi: 10.1002/0471142700.nc0105s10.
This unit presents protocols for the synthesis and characterization of nucleosides with unnatural bases in order to develop bases for the expansion of the genetic alphabet or for nonselective pairing opposite natural bases. Protocols describe the design, synthesis, and characterization of unnatural base pairs involving 1-beta-D-2-deoxyribosyl-N- and -C-nucleosides. Determination of the thermodynamic and kinetic parameters of unnatural nucleosides is accomplished by incorporation into oligonucleotides and subsequent evaluation as described herein.
本单元介绍了合成和表征带有非天然碱基的核苷的方案，以开发用于扩展遗传字母表的碱基，或用于与天然碱基进行非选择性配对的碱基。方案描述了涉及1-β-D-2-脱氧核糖基-N-和-C-核苷的非天然碱基对的设计、合成和表征。通过将非天然核苷掺入寡核苷酸并随后按本文所述进行评估，来确定其热力学和动力学参数。
