Bergstrom Donald E
Purdue University, West Lafayette, Indiana.
Curr Protoc Nucleic Acid Chem. 2009 Jun;Chapter 1:1.4.1-1.4.32. doi: 10.1002/0471142700.nc0104s37.
Synthetic modified nucleosides designed to pair in unusual ways with natural nucleobases have many potential applications in biology and biotechnology. This overview lays the foundation for future protocol units on synthesis and application of unnatural bases, with particular emphasis on unnatural base analogs that mimic natural bases in size, shape, and biochemical processing. Topics covered include base pairs with alternative H-bonding schemes, dimensionally expanded base pairs, hydrophobic base pairs, metal-ligated bases, degenerate bases, universal nucleosides, and triplex constituents.
设计用于以非同寻常的方式与天然核碱基配对的合成修饰核苷在生物学和生物技术领域有许多潜在应用。本综述为未来关于非天然碱基合成与应用的实验方案单元奠定了基础，特别强调了在大小、形状和生化处理方面模拟天然碱基的非天然碱基类似物。涵盖的主题包括具有替代氢键方案的碱基对、尺寸扩展的碱基对、疏水碱基对、金属连接的碱基、简并碱基、通用核苷和三链体成分。
