Taton T Andrew
University of Minnesota, Minneapolis, Minnesota, USA.
Curr Protoc Nucleic Acid Chem. 2002 Aug;Chapter 12:Unit 12.2. doi: 10.1002/0471142700.nc1202s09.
This unit describes the preparation of conjugates between nanometer-scale gold particles and synthetic oligonucleotides. Oligonucleotide-functionalized gold nanoparticles are finding increased use in both the construction of complex, tailored nanostructures and the optimization of DNA sequence analysis. The protocols in this unit outline the synthesis, purification, and characterization of nanoparticle-DNA conjugates for applications in nanotechnology and biotechnology. Separate procedures are presented for nanoparticles functionalized with just one or a few oligonucleotide strands and for nanoparticles functionalized with a dense layer of oligonucleotide strands. The different physical and chemical properties of these two types of conjugates are discussed, as are their stability and utility in different environments.
本单元介绍了纳米级金颗粒与合成寡核苷酸之间缀合物的制备方法。寡核苷酸功能化的金纳米颗粒在构建复杂的定制纳米结构以及优化DNA序列分析方面的应用越来越广泛。本单元中的方案概述了用于纳米技术和生物技术应用的纳米颗粒 - DNA缀合物的合成、纯化和表征。分别介绍了仅用一条或几条寡核苷酸链功能化的纳米颗粒以及用致密寡核苷酸链层功能化的纳米颗粒的制备方法。讨论了这两种类型缀合物的不同物理和化学性质，以及它们在不同环境中的稳定性和实用性。
