用限制性核酸内切酶消化DNA。

Digestion of DNA with restriction endonucleases.

作者信息

Bloch K D

机构信息

Harvard Medical School, Boston, Massachusetts, USA.

出版信息

Curr Protoc Protein Sci. 2001 May;Appendix 4:Appendix 4I. doi: 10.1002/0471140864.psa04is13.

DOI:10.1002/0471140864.psa04is13

PMID:18429084

Abstract

Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. This appendix describes restriction endonucleases and their properties.

摘要

限制性内切核酸酶能够识别短的DNA序列，并在识别序列内或其相邻的特定位点切割双链DNA。将DNA切割成离散片段是分子生物学中最基本的操作之一。本附录介绍了限制性内切核酸酶及其特性。