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限制性内切核酸酶HindII和TaqI可在其识别序列内切割含有错配核苷酸的DNA。

Restriction endonucleases HindII and TaqI cleave DNA with mismatched nucleotides within their recognition sequences.

作者信息

Jiricny J, Martin D

出版信息

Nucleic Acids Res. 1986 Mar 11;14(5):1943-9. doi: 10.1093/nar/14.5.1943.

DOI:10.1093/nar/14.5.1943
PMID:3008080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339633/
Abstract

Restriction endonucleases HindII and TaqI, but not SalI, were found to efficiently cleave synthetic hexadecanucleotide duplexes which contained either an A/C or a G/T mismatch within their respective restriction sites. Double-stranded M13 DNAs with identical mismatches were also cleaved under the assay conditions. These results suggest that the distortion of the DNA duplex, caused by these purine/pyrimidine mismatches is not sufficiently large so as to interfere with the recognition and the subsequent cleavage of the DNA by these two enzymes. HindII and SalI, but not TaqI, were furthermore shown to hydrolyze the two strands of the duplex with different rates. The differences between the mode of recognition of their respective restriction sites by these three enzymes are discussed.

摘要

发现限制性内切酶HindII和TaqI（而非SalI）能够有效切割合成的十六聚体双链体，这些双链体在其各自的限制位点内含有A/C或G/T错配。具有相同错配的双链M13 DNA在检测条件下也会被切割。这些结果表明，由这些嘌呤/嘧啶错配引起的DNA双链体扭曲不够大，以至于不会干扰这两种酶对DNA的识别和随后的切割。此外，还表明HindII和SalI（而非TaqI）以不同速率水解双链体的两条链。讨论了这三种酶对其各自限制位点的识别模式之间的差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aed/339633/540efc202287/nar00274-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aed/339633/540efc202287/nar00274-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aed/339633/540efc202287/nar00274-0027-a.jpg

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3

本文引用的文献

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Structure, dynamics, and energetics of deoxyguanosine . thymidine wobble base pair formation in the self-complementary d(CGTGAATTCGCG) duplex in solution.溶液中自互补双链体d(CGTGAATTCGCG) 中脱氧鸟苷-胸苷摆动碱基对形成的结构、动力学和能量学
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Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs.
限制性内切核酸酶在其被错配碱基对取代的DNA识别序列处进行水解。
Nucleic Acids Res. 1986 Jun 11;14(11):4407-20.
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Some restriction endonucleases tolerate single mismatches of the pyrimidine.purine type.一些限制性内切核酸酶可容忍嘧啶-嘌呤类型的单个错配。
Nucleic Acids Res. 1990 Apr 25;18(8):2159-62. doi: 10.1093/nar/18.8.2159.
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Effects of 2-chloroadenine substitution in DNA on restriction endonuclease cleavage reactions.DNA中2-氯腺嘌呤取代对限制性内切酶切割反应的影响。
Nucleic Acids Res. 1991 Jun 11;19(11):3143-8. doi: 10.1093/nar/19.11.3143.
与EcoRI核酸内切酶形成晶体复合物的扭结DNA。
Nature. 1984;309(5966):327-31. doi: 10.1038/309327a0.
4
Effect of nucleotide analogs on the cleavage of DNA by the restriction enzymes AluI, DdeI, HinfI, RsaI, and TaqI.核苷酸类似物对限制性内切酶AluI、DdeI、HinfI、RsaI和TaqI切割DNA的影响。
J Biol Chem. 1983 Dec 25;258(24):15206-13.
5
Synthesis of deoxyoligonucleotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease Bgl II and Sau 3AI (nucleosides and nucleotides Part 55).含7-脱氮腺嘌呤的脱氧寡核苷酸的合成：限制性内切酶Bgl II和Sau 3AI的识别与切割（核苷与核苷酸 第55部分）
Nucleic Acids Res. 1984 Dec 11;12(23):8939-49. doi: 10.1093/nar/12.23.8939.
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Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site.一种在切割位点含有硫代磷酸酯基团的含EcoRI识别序列的八核苷酸的合成与表征。
Biochemistry. 1984 Jul 17;23(15):3443-53. doi: 10.1021/bi00310a010.
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High-resolution structure of a DNA helix containing mismatched base pairs.包含错配碱基对的DNA螺旋的高分辨率结构。
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Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.一种嗜血杆菌属限制性内切核酸酶对单链DNA的位点特异性切割。
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Use of short synthetic DNA duplexes as substrates for the restriction endonucleases Hpa II and Mno I.使用短的合成DNA双链体作为限制性内切酶Hpa II和Mno I的底物。
J Biol Chem. 1979 Sep 25;254(18):8943-50.