Harvey David J, Dwek Raymond A, Rudd Pauline M
University of Oxford, Oxford, United Kingdom.
Curr Protoc Protein Sci. 2006 Mar;Chapter 12:12.7.1-12.7.18. doi: 10.1002/0471140864.ps1207s43.
Acquisition of mass spectra using fast-atom bombardment (FAB), matrix-assisted laser desorption/ionization (MALDI), and electrospray ionization (ESI) mass spectrometry is summarized in this unit. Molecular weights of the carbohydrates provide information on their composition in terms of isobaric monosaccharide composition, and tables of residue masses are provided to assist these calculations. More detailed structural analysis can be performed by fragmentation; the main fragmentation modes of carbohydrates and their nomenclature are discussed. Fragmentation of negative ions generated by nano-electrospray, in particular, provide very informative spectra and give details such as the position of fucose substitution, branching pattern, and the presence or absence of bisecting GlcNAc residues. Practical details are given for the purification of samples prior to mass spectrometric analysis and for recording FAB, MALDI, and ESI spectra.
本单元总结了使用快原子轰击（FAB）、基质辅助激光解吸/电离（MALDI）和电喷雾电离（ESI）质谱法获取质谱的方法。碳水化合物的分子量可根据等压单糖组成提供其组成信息，并提供了残基质量表以辅助这些计算。通过碎片化可进行更详细的结构分析；讨论了碳水化合物的主要碎片化模式及其命名法。特别是通过纳米电喷雾产生的负离子碎片化，可提供非常有信息量的光谱，并给出诸如岩藻糖取代位置、分支模式以及是否存在平分型N-乙酰葡糖胺残基等细节。给出了质谱分析前样品纯化以及记录FAB、MALDI和ESI光谱的实际细节。
