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携带来自噬菌体λ的操纵基因的缺陷型猿猴病毒40基因组的结构

Structure of a defective simian virus 40 genome bearing an operator from bacteriophage lambda.

作者信息

Davoli D, Nussbaum A L, Fareed G C

出版信息

J Virol. 1976 Sep;19(3):1100-6. doi: 10.1128/JVI.19.3.1100-1106.1976.

DOI:10.1128/JVI.19.3.1100-1106.1976
PMID:184298
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354950/
Abstract

We examined further the physical structure of the simian virus 40 (SV40) and bacteriophage lambda DNA sequences in an SV40-lambda hybrid that had been propagated in monkey kidney cells. The SV40 vector portion of the hybrid, which was a small fragment isolated from a reiteration mutant of SV40, contained the site for initiation of SV40 DNA replication. Electron microscope heteroduplex and restriction endonuclease analyses revealed a tandem duplication of the SV40 vector segment linked to a 2,300-base pair portion (lambda map units 71 to 76) of the lambda immunity region. The defective hybrid genome thus harbors two origins for SV40 DNA replication in addition to the leftward operator and the N gene of lambda.

摘要

我们进一步研究了在猴肾细胞中增殖的猿猴病毒40（SV40）-噬菌体λ杂种中SV40和噬菌体λ DNA序列的物理结构。该杂种的SV40载体部分是从SV40的一个重复突变体中分离出的小片段，包含SV40 DNA复制起始位点。电子显微镜异源双链分析和限制性内切酶分析显示，SV40载体片段串联重复，并与λ免疫区域的一个2300碱基对部分（λ图谱单位71至76）相连。因此，有缺陷的杂种基因组除了λ的向左操纵子和N基因外，还含有两个SV40 DNA复制起点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/ad56c8a23bb7/jvirol00225-0353-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/3b14b7a293f2/jvirol00225-0349-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/392f1d63219e/jvirol00225-0351-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/33d0fbca77ce/jvirol00225-0352-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/ad56c8a23bb7/jvirol00225-0353-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/3b14b7a293f2/jvirol00225-0349-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/392f1d63219e/jvirol00225-0351-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/33d0fbca77ce/jvirol00225-0352-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9ad/354950/ad56c8a23bb7/jvirol00225-0353-a.jpg

相似文献

1
Structure of a defective simian virus 40 genome bearing an operator from bacteriophage lambda.携带来自噬菌体λ的操纵基因的缺陷型猿猴病毒40基因组的结构
J Virol. 1976 Sep;19(3):1100-6. doi: 10.1128/JVI.19.3.1100-1106.1976.
2
Construction and propagation of a defective simian virus 40 genome bearing an operator from bacteriophage lambda.
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1068-72. doi: 10.1073/pnas.73.4.1068.
3
Propagation of a segment of bacteriophage lamda-DNA in monkey cells after covalent linkage to a defective simian virus 40 genome.一段噬菌体λ-DNA与缺陷型猿猴病毒40基因组共价连接后在猴细胞中的增殖。
Cell. 1976 Mar;7(3):349-59. doi: 10.1016/0092-8674(76)90164-1.
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Construction of hybrid viruses containing SV40 and lambda phage DNA segments and their propagation in cultured monkey cells.含有SV40和λ噬菌体DNA片段的杂交病毒的构建及其在培养的猴细胞中的增殖。
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Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VII. Characterization of the simian virus 40 RNA species induced by five nondefective hybrid viruses.无缺陷腺病毒2-猴病毒40杂交病毒的研究。VII. 五种无缺陷杂交病毒诱导的猴病毒40 RNA种类的特性
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DNA sequences similar to those around the simian virus 40 origin of replication are present in the monkey genome.与猿猴病毒40复制起点周围的序列相似的DNA序列存在于猴子基因组中。
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Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus, Ad2 + ND 1 .非缺陷型腺病毒2-猴病毒40杂交病毒的研究。IV. 野生型猴病毒40和非缺陷型杂交病毒Ad2+ND1诱导的猴病毒40核糖核酸种类的特性
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Transduction of a bacterial gene into mammalian cells.将细菌基因导入哺乳动物细胞。
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In situ hybridization of repetitive monkey genome sequences isolated from defective simian virus 40 DNA.
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引用本文的文献

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Molecular cloning of DNA. An introduction into techniques and problems.DNA的分子克隆：技术与问题导论
Hum Genet. 1977 Dec 29;40(1):1-72. doi: 10.1007/BF00280831.
2
Transduction of a bacterial gene into mammalian cells.将细菌基因导入哺乳动物细胞。
Proc Natl Acad Sci U S A. 1978 May;75(5):2117-21. doi: 10.1073/pnas.75.5.2117.

本文引用的文献

1
Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.对噬菌体λ中的限制靶点进行操作，以形成用于DNA片段的受体染色体。
Nature. 1974 Oct 11;251(5475):476-81. doi: 10.1038/251476a0.
2
Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes.信函：关于细菌宿主修饰与限制系统及其酶的一种建议命名法。
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3
Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.
用核酸内切酶RI对λ噬菌体DNA切割产生的DNA片段进行图谱绘制。
Nature. 1973 Jan 12;241(5385):120-3. doi: 10.1038/241120a0.
4
Separation and analysis of promoter sites in bacteriophage lambda DNA by specific endonucleases.用特异性核酸内切酶对噬菌体λDNA中的启动子位点进行分离和分析。
J Mol Biol. 1974 Jan 5;85(4):475-84. doi: 10.1016/0022-2836(74)90310-6.
5
Characterization of a rearrangement in viral DNA: mapping of the circular simian virus 40-like DNA containing a triplication of a specific one-third of the viral genome.病毒DNA重排的特征分析：含病毒基因组特定三分之一区域三倍体的环状猴病毒40样DNA的图谱绘制
J Mol Biol. 1974 Aug 5;87(2):289-301. doi: 10.1016/0022-2836(74)90150-8.
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Triplication of a unique genetic segment in a simian virus 40-like virus of human origin and evolution of new viral genomes.
J Mol Biol. 1974 Aug 5;87(2):275-88. doi: 10.1016/0022-2836(74)90149-1.
7
Conversion of closed circular DNA molecules to single-nicked molecules by digestion with DNAase I in the presence of ethidium bromide.在溴化乙锭存在的情况下，用DNA酶I消化将闭环DNA分子转化为单切口分子。
Biochim Biophys Acta. 1975 Oct 15;407(3):365-75. doi: 10.1016/0005-2787(75)90104-5.
8
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.用EcoRI限制性内切酶切割噬菌体λ DNA的研究。
J Mol Biol. 1975 Jan 25;91(3):315-28. doi: 10.1016/0022-2836(75)90383-6.
9
Propagation of a segment of bacteriophage lamda-DNA in monkey cells after covalent linkage to a defective simian virus 40 genome.一段噬菌体λ-DNA与缺陷型猿猴病毒40基因组共价连接后在猴细胞中的增殖。
Cell. 1976 Mar;7(3):349-59. doi: 10.1016/0092-8674(76)90164-1.
10
Construction and propagation of a defective simian virus 40 genome bearing an operator from bacteriophage lambda.
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1068-72. doi: 10.1073/pnas.73.4.1068.