Davoli D, Nussbaum A L, Fareed G C
J Virol. 1976 Sep;19(3):1100-6. doi: 10.1128/JVI.19.3.1100-1106.1976.
We examined further the physical structure of the simian virus 40 (SV40) and bacteriophage lambda DNA sequences in an SV40-lambda hybrid that had been propagated in monkey kidney cells. The SV40 vector portion of the hybrid, which was a small fragment isolated from a reiteration mutant of SV40, contained the site for initiation of SV40 DNA replication. Electron microscope heteroduplex and restriction endonuclease analyses revealed a tandem duplication of the SV40 vector segment linked to a 2,300-base pair portion (lambda map units 71 to 76) of the lambda immunity region. The defective hybrid genome thus harbors two origins for SV40 DNA replication in addition to the leftward operator and the N gene of lambda.
我们进一步研究了在猴肾细胞中增殖的猿猴病毒40(SV40)-噬菌体λ杂种中SV40和噬菌体λ DNA序列的物理结构。该杂种的SV40载体部分是从SV40的一个重复突变体中分离出的小片段,包含SV40 DNA复制起始位点。电子显微镜异源双链分析和限制性内切酶分析显示,SV40载体片段串联重复,并与λ免疫区域的一个2300碱基对部分(λ图谱单位71至76)相连。因此,有缺陷的杂种基因组除了λ的向左操纵子和N基因外,还含有两个SV40 DNA复制起点。