Tanaka Y, Yamaguchi A, Fujikawa T, Sakuma K, Morita I, Ishii K
Laboratory of Human Performance and Fitness, Graduate School of Education, Hokkaido University, Sapporo, Hokkaido, Japan.
Acta Physiol (Oxf). 2008 Oct;194(2):149-59. doi: 10.1111/j.1748-1716.2008.01866.x. Epub 2008 Apr 19.
To examine the relations between specific fibroblast growth factors (FGFs) and satellite cell activation during muscle regeneration and hypertrophy in vivo, we measured mRNA expression of FGFs and myogenic markers in rat plantaris muscle after bupivacaine administration and synergist ablation.
mRNA levels for MyoD, myogenin, proliferating cell nuclear antigen (PCNA), p21, M-cadherin, Pax7, FGF-1, FGF-2, FGF-3, FGF-4, FGF-5, FGF-6, FGF-7, FGF-8 and hepatocyte growth factor (HGF) were measured continually for up to 72 h after bupivacaine administration and synergist ablation. FGF-5, FGF-7 and HGF proteins were immunostained at 72 h after bupivacaine administration.
MyoD and PCNA mRNAs started increasing 24 h after bupivacaine administration. Myogenin, p21, M-cadherin and Pax7 mRNAs started to increase after 48 and 72 h. After synergist ablation, MyoD, PCNA, M-cadherin and Pax7 mRNAs had increased at 24 and 48 h, and myogenin and p21 mRNAs at 12 and 24 h. FGF-1, FGF-7 and HGF mRNAs after the treatments started to increase at the same time as MyoD and PCNA mRNAs. FGF-5 was expressed at the same time as MyoD and PCNA mRNAs after bupivacaine administration but did not after the ablation. FGF-2, FGF-3, FGF-4, FGF-6 and FGF-8 mRNAs were not associated with the expression of the myogenic markers. FGF-7 and HGF proteins were expressed in immature muscle fibre nuclei and the extracellular matrix, but FGF-5 protein was preferentially expressed in extracellular matrix.
These results indicate that FGF-1, FGF-7 and HGF are associated with specific myogenic marker expression during muscle regeneration and hypertrophy.
为了研究在体内肌肉再生和肥大过程中特定成纤维细胞生长因子（FGFs）与卫星细胞激活之间的关系，我们测量了布比卡因给药和协同肌切除后大鼠比目鱼肌中FGFs和成肌标志物的mRNA表达。
在布比卡因给药和协同肌切除后持续72小时测量MyoD、肌细胞生成素、增殖细胞核抗原（PCNA）、p21、M-钙黏蛋白、Pax7、FGF-1、FGF-2、FGF-3、FGF-4、FGF-5、FGF-6、FGF-7、FGF-8和肝细胞生长因子（HGF）的mRNA水平。在布比卡因给药72小时后对FGF-5、FGF-7和HGF蛋白进行免疫染色。
布比卡因给药后24小时，MyoD和PCNA mRNA开始增加。肌细胞生成素、p21、M-钙黏蛋白和Pax7 mRNA在48和72小时后开始增加。协同肌切除后，MyoD、PCNA、M-钙黏蛋白和Pax7 mRNA在24和48小时增加，肌细胞生成素和p21 mRNA在12和24小时增加。处理后的FGF-1、FGF-7和HGF mRNA与MyoD和PCNA mRNA同时开始增加。布比卡因给药后FGF-5与MyoD和PCNA mRNA同时表达，但切除后不表达。FGF-2、FGF-3、FGF-4、FGF-6和FGF-8 mRNA与成肌标志物的表达无关。FGF-7和HGF蛋白在未成熟肌纤维核和细胞外基质中表达，但FGF-5蛋白优先在细胞外基质中表达。
这些结果表明，FGF-1、FGF-7和HGF在肌肉再生和肥大过程中与特定的成肌标志物表达相关。
