Tanaka Shoji, Kawahara Ei, Nakagawa Takao
Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Japan.
J Phys Ther Sci. 2015 Jul;27(7):2349-52. doi: 10.1589/jpts.27.2349. Epub 2015 Jul 22.
[Purpose] The present study aimed to determine the effects of short muscle strength exercise on hepatocyte growth factor expression and satellite cell activation. [Subjects] The study included 72 2-12-week-old male Sprague-Dawley rats. [Methods] The rat plantaris muscle was contracted with a 5-min electrical stimulation of the sciatic nerve, and then, the mRNA expressions of hepatocyte growth factor and myogenic regulatory factors in the plantaris muscle were determined, and the phosphorylation of the hepatocyte growth factor receptor (c-Met) was examined. [Results] The mRNA expressions of hepatocyte growth factor and myogenic regulatory factors increased after a short muscle contraction compared to that un-contraction. Immunofluorescence analysis showed the expression of hepatocyte growth factor protein and the possibility that downstream biological changes occurred in the hepatocyte growth factor-bound c-Met. [Conclusion] Our results demonstrated that activation of satellite cells induced hepatocyte growth factor expression during muscle contraction with a short 5-min electrical stimulation, which simulates short muscle strength exercise in physical therapy. The present study provides evidence for the use of short muscle strength exercise in physical therapy.
[目的] 本研究旨在确定短期肌肉力量锻炼对肝细胞生长因子表达及卫星细胞激活的影响。[对象] 本研究纳入了72只2至12周龄的雄性斯普拉格-道利大鼠。[方法] 对大鼠的比目鱼肌进行5分钟坐骨神经电刺激使其收缩，然后测定比目鱼肌中肝细胞生长因子及生肌调节因子的mRNA表达，并检测肝细胞生长因子受体（c-Met）的磷酸化情况。[结果] 与未收缩相比，短期肌肉收缩后肝细胞生长因子及生肌调节因子的mRNA表达增加。免疫荧光分析显示了肝细胞生长因子蛋白的表达以及肝细胞生长因子结合的c-Met下游发生生物学变化的可能性。[结论] 我们的结果表明，在5分钟的短时间电刺激肌肉收缩过程中，卫星细胞的激活诱导了肝细胞生长因子的表达，该电刺激模拟了物理治疗中的短期肌肉力量锻炼。本研究为在物理治疗中使用短期肌肉力量锻炼提供了证据。
