通过声穿孔进行基因转导。

Gene transduction by sonoporation.

作者信息

Ohta Sho, Suzuki Kentaro, Ogino Yukiko, Miyagawa Shinichi, Murashima Aki, Matsumaru Daisuke, Yamada Gen

机构信息

Department of Neurobiology and Anatomy, School of Medicine, University of Utah, Salt Lake City, Utah, USA.

出版信息

Dev Growth Differ. 2008 Aug;50(6):517-20. doi: 10.1111/j.1440-169X.2008.01026.x. Epub 2008 Apr 22.

DOI:10.1111/j.1440-169X.2008.01026.x

PMID:18430029

Abstract

Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.

摘要

基因转导技术是理解胚胎发育潜在基因功能或基因级联反应的重要工具。在本综述中，我们介绍一种利用微泡和超声的基因转导方法（以下简称声孔效应法）。声孔效应法操作相对简单，能轻松将基因导入间充质细胞，且对靶组织无明显损伤。因此，声孔效应法在基因转导以研究形态发生的分子机制方面是有效的。