Liu Y, Yang H, Sakanishi A
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu.
Biotechnol Adv. 2006 Jan-Feb;24(1):1-16. doi: 10.1016/j.biotechadv.2005.04.002. Epub 2005 Jun 1.
Development of nonviral gene transfer methods would be a valuable alternative of gene therapy or transformation. Ultrasound can produce a variety of nonthermal bioeffects via acoustic cavitation. Cavitation bubbles can induce cell death or transient membrane permeabilization (sonoporation) on cells. Application of sonoporation for gene transfer into cells or tissues develops quickly in recent years. Many studies have been performed in vitro exposure systems to a variety of cell lines transfected successfully. In vivo, cavitation initiation and control are more difficult, but can be enhanced by ultrasound contrast agents (microbubbles). The use of ultrasound for nonviral gene delivery has been applied for mammalian systems, which provides a fundamental basis and strong promise for development of new gene therapy methods for clinical medicine. In this paper, ultrasound applied to plant cell transformation or gene transfer is reviewed. Recently, most researches are focused on sonication-assisted Agrobacterium-mediated transformation (SAAT) in plant cells or tissues. Microbubbles are also proposed to apply to gene transfer in plant cells and tissues.
非病毒基因转移方法的开发将成为基因治疗或转化的一种有价值的替代方法。超声波可通过声空化产生多种非热生物效应。空化气泡可诱导细胞死亡或使细胞产生瞬时膜通透性（声孔效应）。近年来，声孔效应在细胞或组织基因转移中的应用发展迅速。许多研究已在体外暴露系统中对多种细胞系成功进行了转染。在体内，空化的引发和控制更为困难，但可通过超声造影剂（微泡）得到增强。超声用于非病毒基因递送已应用于哺乳动物系统，这为临床医学新基因治疗方法的开发提供了基本依据和有力前景。本文综述了超声在植物细胞转化或基因转移中的应用。最近，大多数研究集中在植物细胞或组织中的超声辅助农杆菌介导转化（SAAT）。微泡也被提议应用于植物细胞和组织的基因转移。
