Cannata Stefano M, Bernardini Sergio, Filoni Sergio, Gargioli Cesare
Department of Biology, Tor Vergata University, Via della Ricerca Scientifica, 00133 Rome, Italy.
J Anat. 2008 May;212(5):621-6. doi: 10.1111/j.1469-7580.2008.00886.x.
The outer cornea and pericorneal epidermis (lentogenic area) of larval Xenopus laevis are the only epidermal regions competent to regenerate a lens under the influence of the retinal inducer. However, the head epidermis of the lentogenic area can acquire the lens-regenerating competence following transplantation of an eye beneath it. In this paper we demonstrate that both the outer cornea and the head epidermis covering a transplanted eye are capable of responding not only to the retinal inducer of the larval eye but also to the inductive action of the embryonic optic vesicle by synthesizing crystallins. As the optic vesicle is a very weak lens inductor, which promotes crystallin synthesis only on the lens biased ectoderm of the embryo, these results indicate that the lens-forming competence in the outer cornea and epidermis of larval X. laevis corresponds to the persistence and acquisition of a condition similar to that of the embryonic biased ectoderm.
非洲爪蟾幼体的角膜外层和角膜周围表皮(晶状体发生区)是仅有的在视网膜诱导物影响下能够再生晶状体的表皮区域。然而,晶状体发生区的头部表皮在其下方移植一只眼睛后能够获得晶状体再生能力。在本文中,我们证明覆盖移植眼的角膜外层和头部表皮不仅能够对幼体眼睛的视网膜诱导物做出反应,还能够通过合成晶状体蛋白对胚胎视泡的诱导作用做出反应。由于视泡是一种非常弱的晶状体诱导物,仅能促进胚胎晶状体偏向外胚层上的晶状体蛋白合成,这些结果表明非洲爪蟾幼体角膜外层和表皮中的晶状体形成能力对应于类似于胚胎偏向外胚层的状态的持续和获得。