Gopinath M, Shaila M S
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Virus Res. 2008 Jul;135(1):150-4. doi: 10.1016/j.virusres.2008.03.005. Epub 2008 Apr 21.
Rinderpest virus belongs to the family of Paramyxoviridae, consisting of non-segmented negative sense RNA viruses. Viral transcription and replication are carried out by the RNA dependent RNA polymerase L protein which functions together with P protein as L-P complex. The exact events triggering the polymerase complex from transcription to replication function is poorly understood. In the present work, an in vitro transcription system has been described with partially purified L-P complex expressed in insect cells and viral genomic RNA. The relative abundance of each species of mRNA synthesized in vitro decreased from the 3' end of the genome to the 5' end similar to their abundance in virus infected cells. Recombinant L-P complex was unable to synthesize leader RNA suggesting the initiation of transcription from gene start site and not at the 3' end of the genome.
牛瘟病毒属于副粘病毒科，由非节段性负链RNA病毒组成。病毒转录和复制由RNA依赖性RNA聚合酶L蛋白完成，该蛋白与P蛋白作为L-P复合物共同发挥作用。引发聚合酶复合物从转录功能转变为复制功能的确切事件尚不清楚。在本研究中，描述了一种体外转录系统，该系统使用在昆虫细胞中表达的部分纯化的L-P复合物和病毒基因组RNA。体外合成的每种mRNA的相对丰度从基因组的3'端到5'端逐渐降低，这与它们在病毒感染细胞中的丰度相似。重组L-P复合物无法合成前导RNA，这表明转录起始于基因起始位点而非基因组的3'端。
