Benziane Boubacar, Chibalin Alexander V
Deptartment of Molecular Medicine and Surgery, Integrative Physiology, Karolinska Institutet 171 77, Stockholm Sweden.
Am J Physiol Endocrinol Metab. 2008 Sep;295(3):E553-8. doi: 10.1152/ajpendo.90261.2008. Epub 2008 Apr 22.
The skeletal muscle sodium pump plays a major role in the removal of K(+) ions from the circulation postprandial, or after a physical activity bout, thereby preventing the development of hyperkalemia and fatigue. Insulin and muscle contractions stimulate Na(+)-K(+)-ATPase activity in skeletal muscle, at least partially via translocation of sodium pump units to the plasma membrane from intracellular stores. The molecular mechanism of this phenomenon is poorly understood. Due to the contradictory reports in the literature, the very existence of the translocation of Na(+)-K(+)-ATPase to the skeletal muscle cell surface is questionable. This review summarizes more than 30 years work on the skeletal muscle sodium pump translocation paradigm. Furthermore, the methodological caveats of major approaches to study the sodium pump translocation in skeletal muscle are discussed. An understanding of the molecular regulation of Na(+)-K(+)-ATPase in skeletal muscle will have important clinical implications for the understanding of the development of complications associated with the metabolic syndrome, such as cardiovascular diseases or increased muscle fatigue in diabetic patients.
骨骼肌钠泵在餐后或体育活动后从循环中清除钾离子方面发挥着重要作用，从而防止高钾血症和疲劳的发生。胰岛素和肌肉收缩至少部分通过将钠泵单位从细胞内储存部位转运到质膜来刺激骨骼肌中的钠钾ATP酶活性。这种现象的分子机制尚不清楚。由于文献中的报道相互矛盾，钠钾ATP酶向骨骼肌细胞表面转运的存在本身就值得怀疑。这篇综述总结了30多年来关于骨骼肌钠泵转运模式的研究工作。此外，还讨论了研究骨骼肌钠泵转运的主要方法的方法学注意事项。了解骨骼肌中钠钾ATP酶的分子调节对于理解与代谢综合征相关的并发症（如心血管疾病或糖尿病患者肌肉疲劳增加）的发生具有重要的临床意义。
