细小病毒B19的变异体：巢式PCR检测的生物信息学评估

Variants of parvovirus B19: bioinformatical evaluation of nested PCR assays.

作者信息

Bergallo Massimiliano, Costa Cristina, Sidoti Francesca, Novelli Mauro, Ponti Renata, Castagnoli Carlotta, Merlino Chiara, Bernengo Maria Grazia, Cavallo Rossana

机构信息

Department of Public Health and Microbiology, Virology Unit, University of Turin, Turin, Italy.

出版信息

Intervirology. 2008;51(2):75-80. doi: 10.1159/000127429. Epub 2008 Apr 22.

DOI:10.1159/000127429

PMID:18431072

Abstract

Variants of parvovirus B19 are currently grouped into three genotypes: 1 (reference B19 strains), 2 and 3. It has been evidenced that isolate K71 of genotype 2 is more prevalent in skin than the conventional B19 genotype 1. In this study we investigated the detection of parvovirus B19 genotypes by using two nested PCRs and evaluating the suitability of these assays by BLAST search of parvovirus isolates. Subsequently, we analyze the present genotypes in skin biopsies. The two nested PCRs employed in this study allow to amplify 41 isolates as confirmed by bioinformatical validation. The molecular epidemiological characterization of our casistics confirmed the presence of isolate K71 in human skin.

摘要

细小病毒B19的变体目前分为三个基因型：1型（参考B19毒株）、2型和3型。已有证据表明，2型分离株K71在皮肤中的流行率高于传统的B19 1型基因型。在本研究中，我们使用两种巢式PCR检测细小病毒B19基因型，并通过对细小病毒分离株进行BLAST搜索来评估这些检测方法的适用性。随后，我们分析了皮肤活检中的现有基因型。本研究中使用的两种巢式PCR能够扩增41个分离株，经生物信息学验证得到确认。我们病例的分子流行病学特征证实了人类皮肤中存在分离株K71。