Freitas Ronaldo B, Melo Fernando L, Oliveira Darleise S, Romano Camila M, Freitas Maria Rute C, Macêdo Olinda, Linhares Alexandre C, de A Zanotto Paolo M, Durigon Edison Luiz
Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Rodovia BR 316-Km 7, S/N Levilândia, 67037-000 Ananindeua, Pará, Brazil.
J Clin Virol. 2008 Sep;43(1):60-5. doi: 10.1016/j.jcv.2008.03.033. Epub 2008 May 27.
Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations.
To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients in the Amazon region with various clinical presentations.
A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested using specific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction.
B19 DNA was detected in 117 (24%) of 487 samples. Of these, 106 (91%) isolates were genotype 1 and 11 (9%) were genotype 3. No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may have been transmitted via transfusion of blood products.
We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years.
自1990年以来，人细小病毒B19在亚马逊地区流行，与多种临床表现相关。
评估亚马逊地区有各种临床表现的患者中细小病毒B19感染的患病率以及细小病毒B19基因型的相对频率。
使用特异性IgM和IgG抗体检测（ELISA）以及用于病毒DNA检测的PCR对从有细小病毒感染症状的患者中获得的487份临床样本进行检测。通过系统发育重建对部分VP1和VP2区域进行测序和基因分型。
在487份样本中的117份（24%）中检测到B19 DNA。其中，106份（91%）分离株为1型，11份（9%）为3型。未发现2型。1型有三个簇（A1、A2和B），所有3型序列均为3b亚型。1型B簇内所有血液系统疾病患者均感染同一B19谱系，提示该B19谱系可能通过血液制品输血传播。
我们报告了在过去10年中，1型和3b型两种基因型，以及三个1型簇在亚马逊地区共同流行情况。
