van Eden Willem, Wagenaar-Hilbers Josee P A, Wauben Marca H M
Institute of Infectious Diseases and Immunology, Utrecht, The Netherlands.
Curr Protoc Immunol. 2001 May;Chapter 15:15.4.1-15.4.8. doi: 10.1002/0471142735.im1504s19.
Adjuvant arthritis (AA) is an induced form of (sub)chronic arthritis. Strains of rats have a varying genetic susceptibility to AA, whereas mice generally are not susceptible. AA is most easily induced with mycobacteria suspended in oil, although in some strains of rats it can be induced with oily adjuvants in the absence of mycobacteria. The disease is a T cell-mediated autoimmune arthritis that is frequently used to study immunological aspects of rheumatoid arthritis (RA) and other arthritic or inflammatory diseases in humans. Furthermore, it is used as a model for developing and testing antiinflammatory drugs. There are no particularly well-defined autoantigens in AA; in this respect, the model resembles spontaneous arthritic diseases in humans. In all susceptible rat strains, the inflammatory process of AA is self remitting, although usually the disease is severe and leads to permanent joint malformations, including ankylosis; a time line for AA development is presented. This unit describes the induction and evaluation of AA and the preparation of adjuvant used to induce AA.
佐剂性关节炎（AA）是一种诱发型（亚）慢性关节炎。不同品系的大鼠对AA的遗传易感性各不相同，而小鼠通常不易感。用悬浮于油中的分枝杆菌最易诱发AA，不过在某些大鼠品系中，无分枝杆菌时用油性佐剂也可诱发。该疾病是一种T细胞介导的自身免疫性关节炎，常用于研究人类类风湿关节炎（RA）及其他关节炎或炎症性疾病的免疫学方面。此外，它还被用作开发和测试抗炎药物的模型。AA中没有特别明确的自身抗原；在这方面，该模型类似于人类的自发性关节炎疾病。在所有易感大鼠品系中，AA的炎症过程会自行缓解，尽管通常疾病较为严重并会导致永久性关节畸形，包括关节强直；文中给出了AA发展的时间线。本单元描述了AA的诱导和评估以及用于诱导AA的佐剂的制备。
