Vogel S N, Friedman R M, Hogan M M
Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
Curr Protoc Immunol. 2001 May;Chapter 6:Unit 6.9. doi: 10.1002/0471142735.im0609s37.
The Basic Protocol in this unit describes an assay for murine IFN-induced antiviral activity and employs vesicular stomatitis virus (VSV) and IFN-sensitive fibroblasts. Support Protocol describes the preparation of VSV cultures and the calculation of multiplicity of infection (MOI; i.e., concentration of viral particles required to infect cells). Support Protocol describes the antibody neutralization assay, which can be used to identify the bioactive species of IFN in a sample, or to test the potency of an antibody preparation against a particular species of IFN. Alternate Protocol 1 covers measurement of human IFN-induced antiviral activity; while the steps are quite similar to Basic Protocol, different viral and cell cultures are described.
本单元的基本方案描述了一种检测小鼠干扰素诱导抗病毒活性的方法，该方法使用水疱性口炎病毒（VSV）和对干扰素敏感的成纤维细胞。支持方案描述了VSV培养物的制备以及感染复数（MOI，即感染细胞所需的病毒颗粒浓度）的计算。支持方案描述了抗体中和试验，该试验可用于鉴定样品中干扰素的生物活性种类，或检测针对特定种类干扰素的抗体制剂的效力。替代方案1涵盖了人干扰素诱导抗病毒活性的测量；虽然步骤与基本方案非常相似，但描述了不同的病毒和细胞培养物。
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