用于同时测量mRNA表达的多探针核糖核酸酶保护分析
Multiprobe ribonuclease protection assay for simultaneous measurement of mRNA expression.
作者信息
Young Howard A, Subleski Jeffrey J, Krebs Stephanie M
机构信息
National Cancer Institute, Frederick, Maryland, USA.
出版信息
Curr Protoc Immunol. 2003 Aug;Chapter 10:Unit 10.29. doi: 10.1002/0471142735.im1029s54.
PMID:18432896
Abstract
The multiprobe RNase protection assay enables investigators to monitor RNA expression of 8-12 genes with as little as 1 microg of total RNA. The commercial availability of numerous multi-gene template sets makes this assay practical for all basic research programs.
摘要
多探针核糖核酸酶保护分析使研究人员能够用低至1微克的总RNA监测8至12个基因的RNA表达。众多多基因模板集的商业可得性使该分析适用于所有基础研究项目。
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