Young Howard A
Laboratory of Experimental Immunology, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD, USA.
Methods Mol Biol. 2009;511:85-105. doi: 10.1007/978-1-59745-447-6_4.
The ability to monitor gene expression in experimental and clinical samples is an essential element of modern molecular biology and cell biology research. However with the advent of a systems biology approach toward understanding cell and cancer biology, analysis of expression of a single gene is no longer desirable. Today, multiplex analysis, where the expression of 8-100 genes can be monitored in one sample, has become a routine aspect of gene expression analysis. In this chapter the various assays systems commercially available for multiplex analysis of both RNA and protein will be discussed.
监测实验样本和临床样本中基因表达的能力是现代分子生物学和细胞生物学研究的基本要素。然而，随着采用系统生物学方法来理解细胞和癌症生物学，对单个基因表达的分析已不再可取。如今，在一个样本中可监测8至100个基因表达的多重分析已成为基因表达分析的常规内容。在本章中，将讨论可用于RNA和蛋白质多重分析的各种商业检测系统。
