van Aalst John A, Reed Courtney R, Han Li, Andrady Tony, Hromadka Michael, Bernacki Susan, Kolappa Kamalkumar, Collins James B, Loboa Elizabeth G
Division of Plastic and Reconstructive Surgery, University of North Carolina, Chapel Hill, NC 27514, USA.
Ann Plast Surg. 2008 May;60(5):577-83. doi: 10.1097/SAP.0b013e318168db3e.
Nanofibers are an emerging scaffold for tissue engineering. To date no one has reported cell incorporation into nanofibers. Human foreskin fibroblasts and human adipose-derived adult stem cells (hADAS) were grown to confluence, resuspended in phosphate-buffered saline, and then solubilized in polyvinyl alcohol (PVA). Nanofibers were created using an electrospinning technique across an electric potential of 20 kV. Cell interaction with nanofibers was assessed with optical microscopic imaging and scanning electron microscopy. PVA nanofibers with incorporated cells were then solubilized in phosphate-buffered saline; cell viability was assessed by trypan blue exclusion. Viable cells were allowed to proliferate. Chondrogenesis in fibroblasts was induced with TGF-beta1. Both fibroblasts and hADAS survived the electrospinning process and were incorporated into PVA nanofibers. hADAS cell proliferation was negligible; however, fibroblasts proliferated and showed retained ability to undergo chondrogenesis. Cells can be incorporated into nanofibers, with maintained viability, proliferation, and function.
纳米纤维是一种新兴的组织工程支架。迄今为止，尚无关于细胞融入纳米纤维的报道。将人包皮成纤维细胞和人脂肪来源的成体干细胞（hADAS）培养至汇合状态，重悬于磷酸盐缓冲盐水中，然后溶解于聚乙烯醇（PVA）中。使用静电纺丝技术在20 kV的电势下制备纳米纤维。通过光学显微镜成像和扫描电子显微镜评估细胞与纳米纤维的相互作用。然后将含有细胞的PVA纳米纤维溶解于磷酸盐缓冲盐水中；通过台盼蓝排斥法评估细胞活力。使活细胞增殖。用转化生长因子-β1诱导成纤维细胞向软骨生成分化。成纤维细胞和hADAS细胞均在静电纺丝过程中存活并融入PVA纳米纤维中。hADAS细胞增殖可忽略不计；然而，成纤维细胞增殖并表现出保留的软骨生成能力。细胞能够融入纳米纤维中，并保持活力、增殖能力和功能。
