Nol P, Palmer M V, Waters W R, Aldwell F E, Buddle B M, Triantis J M, Linke L M, Phillips G E, Thacker T C, Rhyan J C, Dunbar M R, Salman M D
USDA/APHIS National Wildlife Research Center, Fort Collins, Colorado 80521, USA.
J Wildl Dis. 2008 Apr;44(2):247-59. doi: 10.7589/0090-3558-44.2.247.
We investigated the efficacy of oral and parenteral Mycobacterium bovis bacille Calmette-Guerin Danish strain 1331 (BCG) in its ability to protect white-tailed deer (Odocoileus virginianus) against disease caused by M. bovis infection. Twenty-two white-tailed deer were divided into four groups. One group (n=5) received 10(9) colony-forming units (cfu) BCG via a lipid-formulated oral bait; one group (n=5) received 10(9) cfu BCG in culture directly to the oropharynx, one group (n=6) was vaccinated with 10(6) cfu BCG subcutaneously, and one group served as a control and received culture media directly to the oropharynx (n=6). All animals were challenged 3 mo after vaccination. Five months postchallenge the animals were examined for lesions. Results indicate that both oral forms of BCG and parenterally administered BCG offered significant protection against M. bovis challenge as compared to controls. This study suggests that oral BCG vaccination may be a feasible means of controlling bovine tuberculosis in wild white-tailed deer populations.
我们研究了口服和注射用牛分枝杆菌卡介苗丹麦菌株1331（BCG）保护白尾鹿（弗吉尼亚鹿）免受牛分枝杆菌感染所致疾病的能力。22只白尾鹿被分为四组。一组（n = 5）通过脂质配方口服诱饵接受10⁹菌落形成单位（cfu）的BCG；一组（n = 5）将10⁹ cfu的BCG培养物直接接种到口咽部，一组（n = 6）皮下接种10⁶ cfu的BCG，一组作为对照，将培养基直接接种到口咽部（n = 6）。所有动物在接种疫苗3个月后受到攻击。攻击后5个月检查动物是否有病变。结果表明，与对照组相比，两种口服形式的BCG和注射用BCG都对白尾鹿抵抗牛分枝杆菌攻击提供了显著保护。这项研究表明，口服BCG疫苗接种可能是控制野生白尾鹿种群中牛结核病的一种可行方法。
