Ponczek M B, Nowak P, Wachowicz B
Department of General Biochemistry, University of Lodz, Banacha 12/16, 90 273 Lodz, Poland.
Gen Physiol Biophys. 2008 Mar;27(1):55-8.
The effect of nitronium ion on nitration, carbonylation and coagulation of human fibrinogen (Fg) in vitro was investigated. We observed that nitration of tyrosine, induced by NO2BF4 (0.01 mmol/l), was increased. No changes in carbonylation by NO2BF4 (0.01 mmol/l) were noticed. Mentioned alterations were associated with amplified coagulation of Fg. Higher concentrations of NO2BF4 (1 and 0.1 mmol/l) triggered growth of nitration and carbonylation of Fg, but led to inhibition of polymerization. Slight nitration may be responsible for increase, whereas sizable nitration and oxidation may lead to inhibition of Fg coagulation.
研究了硝鎓离子对人纤维蛋白原（Fg）体外硝化、羰基化和凝血的影响。我们观察到，由四氟硼酸硝（0.01 mmol/l）诱导的酪氨酸硝化增加。未注意到四氟硼酸硝（0.01 mmol/l）引起的羰基化有变化。上述改变与Fg凝血增强有关。更高浓度的四氟硼酸硝（1和0.1 mmol/l）引发Fg硝化和羰基化增加，但导致聚合抑制。轻微硝化可能导致增加，而大量硝化和氧化可能导致Fg凝血抑制。
