通过对祖先碳水化合物附着位点进行替换，反刍动物胰腺核糖核酸酶中糖基化的选择淘汰。
Selection against glycosylation in ruminant pancreatic ribonucleases by replacements in the ancestral carbohydrate attachment site.
作者信息
Gautschi Matthias, Beintema Jaap J
机构信息
Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH-Hönggerberg, HPKE 14, 8093, Zurich, Switzerland.
出版信息
Biochem Genet. 2008 Aug;46(7-8):446-50. doi: 10.1007/s10528-008-9162-x. Epub 2008 Apr 24.
A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn-Met-Thr at residues 34-36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells.
25年前提出的一个假说得到了实验证实，该假说认为反刍动物胰腺核糖核酸酶中存在针对糖基化的选择作用，其方式是在第34 - 36位残基的祖先碳水化合物连接位点天冬酰胺-蛋氨酸-苏氨酸（Asn-Met-Thr）处将蛋氨酸替换为亮氨酸。在牛核糖核酸酶中，第35位的亮氨酸被蛋氨酸取代后，在中国仓鼠卵巢细胞中表达时，糖基化相对增加了三倍。
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